Quantitative mass spectrometry reveals the epigenome as a target of arsenic

Feixia Chu; Xuefeng Ren; Amanda Chasse; Taylor Hickman; Luoping Zhang; Jessica Yuh; Martyn T. Smith; Alma L. Burlingame

doi:10.1016/j.cbi.2010.11.003

Quantitative mass spectrometry reveals the epigenome as a target of arsenic

Feixia Chu
, Xuefeng Ren
, Amanda Chasse
, Taylor Hickman
, Luoping Zhang
, Jessica Yuh
, Martyn T. Smith
, Alma L. Burlingame

Department of Epidemiology and Environmental Health

University of New Hampshire
University of California at Berkeley
University of California at San Francisco

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Recent studies reveal that posttranslational modifications on chromatin proteins, especially histones, organize genomic DNA and mediate various cellular responses to environmental influences. Quantitative mass spectrometric analysis is a powerful approach to reveal these dynamic events on chromatin in a systematic manner. Here, the effects of arsenic exposure on histone epigenetic state were investigated in human UROtsa cells, and a reduction in acetylation level on several histone H3 and H4 lysine residues was detected. Furthermore, MYST1 was shown to be the major histone acetyltransferase for H4 Lys16 and protect UROtsa cells from arsenic toxicity.

Original language	English
Pages (from-to)	113-117
Number of pages	5
Journal	Chemico-Biological Interactions
Volume	192
Issue number	1-2
DOIs	https://doi.org/10.1016/j.cbi.2010.11.003
State	Published - Jun 30 2011

Keywords

Arsenic toxicity
Histone acetyltransferase
Histone modifications
Mass spectrometry
Quantitation

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10.1016/j.cbi.2010.11.003

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title = "Quantitative mass spectrometry reveals the epigenome as a target of arsenic",

abstract = "Recent studies reveal that posttranslational modifications on chromatin proteins, especially histones, organize genomic DNA and mediate various cellular responses to environmental influences. Quantitative mass spectrometric analysis is a powerful approach to reveal these dynamic events on chromatin in a systematic manner. Here, the effects of arsenic exposure on histone epigenetic state were investigated in human UROtsa cells, and a reduction in acetylation level on several histone H3 and H4 lysine residues was detected. Furthermore, MYST1 was shown to be the major histone acetyltransferase for H4 Lys16 and protect UROtsa cells from arsenic toxicity.",

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AU - Chu, Feixia

AU - Ren, Xuefeng

AU - Chasse, Amanda

AU - Hickman, Taylor

AU - Zhang, Luoping

AU - Yuh, Jessica

AU - Smith, Martyn T.

AU - Burlingame, Alma L.

PY - 2011/6/30

Y1 - 2011/6/30

N2 - Recent studies reveal that posttranslational modifications on chromatin proteins, especially histones, organize genomic DNA and mediate various cellular responses to environmental influences. Quantitative mass spectrometric analysis is a powerful approach to reveal these dynamic events on chromatin in a systematic manner. Here, the effects of arsenic exposure on histone epigenetic state were investigated in human UROtsa cells, and a reduction in acetylation level on several histone H3 and H4 lysine residues was detected. Furthermore, MYST1 was shown to be the major histone acetyltransferase for H4 Lys16 and protect UROtsa cells from arsenic toxicity.

AB - Recent studies reveal that posttranslational modifications on chromatin proteins, especially histones, organize genomic DNA and mediate various cellular responses to environmental influences. Quantitative mass spectrometric analysis is a powerful approach to reveal these dynamic events on chromatin in a systematic manner. Here, the effects of arsenic exposure on histone epigenetic state were investigated in human UROtsa cells, and a reduction in acetylation level on several histone H3 and H4 lysine residues was detected. Furthermore, MYST1 was shown to be the major histone acetyltransferase for H4 Lys16 and protect UROtsa cells from arsenic toxicity.

KW - Arsenic toxicity

KW - Histone acetyltransferase

KW - Histone modifications

KW - Mass spectrometry

KW - Quantitation

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ER -

Quantitative mass spectrometry reveals the epigenome as a target of arsenic

Abstract

Keywords

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