@inbook{0ffa78b517e14453a6b343ab78507e62,
title = "Quantitative detection of Aspergillus spp. by real-time nucleic acid sequence-based amplification",
abstract = "Rapid and quantitative detection of Aspergillus from clinical samples may facilitate an early diagnosis of invasive pulmonary aspergillosis (IPA). As nucleic acid-based detection is a viable option, we demonstrate that Aspergillus burdens can be rapidly and accurately detected by a novel real-time nucleic acid assay other than qPCR by using the combination of nucleic acid sequence-based amplification (NASBA) and the molecular beacon (MB) technology. Here, we detail a real-time NASBA assay to determine quantitative Aspergillus burdens in lungs and bronchoalveolar lavage (BAL) fl uids of rats with experimental IPA.",
keywords = "Aspergillus burden, Molecular beacon, Nucleic acid sequence-based amplification, Quantitative, Real time",
author = "Yanan Zhao and Perlin, \{David S.\}",
year = "2013",
doi = "10.1007/978-1-62703-257-5\_6",
language = "English",
isbn = "9781627032568",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "83--92",
booktitle = "Fungal Diagnostics",
}