@inbook{b227c74aee5746598dbf07079ab6d989,
title = "Purification of Tannerella forsythia Surface-Layer (S-Layer) Proteins",
abstract = "The objective of this chapter is to provide a detailed purification protocol for the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia. The procedure involves detergent based solubilization of the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography. The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.",
keywords = "Bacterial glycoproteins, O-glycosylation, Periodontitis, Surface-layer, Tannerella forsythia",
author = "Sreedevi Chinthamani and Settem, \{Prasad R.\} and Kiyonobu Honma and Takuma Nakajima and Ashu Sharma",
note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-0939-2\_13",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "135--142",
booktitle = "Methods in Molecular Biology",
}