Pulmonary surfactant-associated polypeptide SP-C in lipid micelles: CD studies of intact SP-C and NMR secondary structure determination of depalmitoyl-SP-C(1-17)

Jan Johansson; Thomas Szyperski; Kurt Wüthrich

doi:10.1016/0014-5793(95)00216-V

Pulmonary surfactant-associated polypeptide SP-C in lipid micelles: CD studies of intact SP-C and NMR secondary structure determination of depalmitoyl-SP-C(1-17)

Jan Johansson
, Thomas Szyperski
, Kurt Wüthrich

Chemistry

Swiss Federal Institute of Technology Zurich

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

The surfactant-associated polypeptide C (SP-C) is a 35-residue lipopolypeptide which is essential for the function of surfactants used for therapy of infant respiratory distress. Modeling based on the recently determined nuclear magnetic resonance (NMR) structure of native SP-C in an organic solvent showed that SP-C could readily insert into fluid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine bilayers. The present paper describes further physical-chemical studies of intact SP-C and its N-terminal 17-residue polypeptide fragment, depalmitoyl-SPC(1-17), in the presence of dodecylphosphocholine micelles. The results obtained provide a link between the NMR solution structure and the behaviour of SP-C in an ordered lipid environment, and thus present new insights for rational design of SP-C analogs for therapeutic purposes.

Original language	English
Pages (from-to)	261-265
Number of pages	5
Journal	FEBS Letters
Volume	362
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(95)00216-V
State	Published - Apr 10 1995

Keywords

Lipid-associated polypeptide
NMR
Polypeptide conformation
Pulmonary surfactant
Surfactant polypeptide-C

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10.1016/0014-5793(95)00216-V

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title = "Pulmonary surfactant-associated polypeptide SP-C in lipid micelles: CD studies of intact SP-C and NMR secondary structure determination of depalmitoyl-SP-C(1-17)",

abstract = "The surfactant-associated polypeptide C (SP-C) is a 35-residue lipopolypeptide which is essential for the function of surfactants used for therapy of infant respiratory distress. Modeling based on the recently determined nuclear magnetic resonance (NMR) structure of native SP-C in an organic solvent showed that SP-C could readily insert into fluid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine bilayers. The present paper describes further physical-chemical studies of intact SP-C and its N-terminal 17-residue polypeptide fragment, depalmitoyl-SPC(1-17), in the presence of dodecylphosphocholine micelles. The results obtained provide a link between the NMR solution structure and the behaviour of SP-C in an ordered lipid environment, and thus present new insights for rational design of SP-C analogs for therapeutic purposes.",

keywords = "Lipid-associated polypeptide, NMR, Polypeptide conformation, Pulmonary surfactant, Surfactant polypeptide-C",

author = "Jan Johansson and Thomas Szyperski and Kurt W{\"u}thrich",

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T1 - Pulmonary surfactant-associated polypeptide SP-C in lipid micelles

T2 - CD studies of intact SP-C and NMR secondary structure determination of depalmitoyl-SP-C(1-17)

AU - Johansson, Jan

AU - Szyperski, Thomas

AU - Wüthrich, Kurt

PY - 1995/4/10

Y1 - 1995/4/10

N2 - The surfactant-associated polypeptide C (SP-C) is a 35-residue lipopolypeptide which is essential for the function of surfactants used for therapy of infant respiratory distress. Modeling based on the recently determined nuclear magnetic resonance (NMR) structure of native SP-C in an organic solvent showed that SP-C could readily insert into fluid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine bilayers. The present paper describes further physical-chemical studies of intact SP-C and its N-terminal 17-residue polypeptide fragment, depalmitoyl-SPC(1-17), in the presence of dodecylphosphocholine micelles. The results obtained provide a link between the NMR solution structure and the behaviour of SP-C in an ordered lipid environment, and thus present new insights for rational design of SP-C analogs for therapeutic purposes.

AB - The surfactant-associated polypeptide C (SP-C) is a 35-residue lipopolypeptide which is essential for the function of surfactants used for therapy of infant respiratory distress. Modeling based on the recently determined nuclear magnetic resonance (NMR) structure of native SP-C in an organic solvent showed that SP-C could readily insert into fluid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine bilayers. The present paper describes further physical-chemical studies of intact SP-C and its N-terminal 17-residue polypeptide fragment, depalmitoyl-SPC(1-17), in the presence of dodecylphosphocholine micelles. The results obtained provide a link between the NMR solution structure and the behaviour of SP-C in an ordered lipid environment, and thus present new insights for rational design of SP-C analogs for therapeutic purposes.

KW - Lipid-associated polypeptide

KW - NMR

KW - Polypeptide conformation

KW - Pulmonary surfactant

KW - Surfactant polypeptide-C

UR - https://www.scopus.com/pages/publications/0028903909

U2 - 10.1016/0014-5793(95)00216-V

DO - 10.1016/0014-5793(95)00216-V

M3 - Article

C2 - 7729509

AN - SCOPUS:0028903909

SN - 0014-5793

VL - 362

SP - 261

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JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Pulmonary surfactant-associated polypeptide SP-C in lipid micelles: CD studies of intact SP-C and NMR secondary structure determination of depalmitoyl-SP-C(1-17)

Abstract

Keywords

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