Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED)

Michael W. Martynowycz; Tamir Gonen

doi:10.1016/j.xpro.2021.100686

Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED)

Michael W. Martynowycz
, Tamir Gonen

Department of Structural Biology

University of California at Los Angeles

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We present an in-depth protocol to reproducibly prepare crystalline lamellae from protein crystals for subsequent microcrystal electron diffraction (MicroED) experiments. This protocol covers typical soluble proteins and membrane proteins embedded in dense media. Following these steps will allow the user to prepare crystalline lamellae for protein structure determination by MicroED. For complete details on the use and execution of this protocol, please refer to Martynowycz et al. (2019a, 2020a).

Original language	English
Article number	100686
Journal	STAR Protocols
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2021.100686
State	Published - Sep 17 2021

Keywords

Cryo-EM
Microscopy
Protein Biochemistry
Structural Biology

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10.1016/j.xpro.2021.100686

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title = "Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED)",

abstract = "We present an in-depth protocol to reproducibly prepare crystalline lamellae from protein crystals for subsequent microcrystal electron diffraction (MicroED) experiments. This protocol covers typical soluble proteins and membrane proteins embedded in dense media. Following these steps will allow the user to prepare crystalline lamellae for protein structure determination by MicroED. For complete details on the use and execution of this protocol, please refer to Martynowycz et al. (2019a, 2020a).",

keywords = "Cryo-EM, Microscopy, Protein Biochemistry, Structural Biology",

author = "Martynowycz, \{Michael W.\} and Tamir Gonen",

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AU - Martynowycz, Michael W.

AU - Gonen, Tamir

PY - 2021/9/17

Y1 - 2021/9/17

N2 - We present an in-depth protocol to reproducibly prepare crystalline lamellae from protein crystals for subsequent microcrystal electron diffraction (MicroED) experiments. This protocol covers typical soluble proteins and membrane proteins embedded in dense media. Following these steps will allow the user to prepare crystalline lamellae for protein structure determination by MicroED. For complete details on the use and execution of this protocol, please refer to Martynowycz et al. (2019a, 2020a).

AB - We present an in-depth protocol to reproducibly prepare crystalline lamellae from protein crystals for subsequent microcrystal electron diffraction (MicroED) experiments. This protocol covers typical soluble proteins and membrane proteins embedded in dense media. Following these steps will allow the user to prepare crystalline lamellae for protein structure determination by MicroED. For complete details on the use and execution of this protocol, please refer to Martynowycz et al. (2019a, 2020a).

KW - Cryo-EM

KW - Microscopy

KW - Protein Biochemistry

KW - Structural Biology

UR - https://www.scopus.com/pages/publications/85111216915

U2 - 10.1016/j.xpro.2021.100686

DO - 10.1016/j.xpro.2021.100686

M3 - Article

C2 - 34382014

AN - SCOPUS:85111216915

SN - 2666-1667

VL - 2

JO - STAR Protocols

JF - STAR Protocols

IS - 3

M1 - 100686

ER -

Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED)

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Keywords

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