Proteomic analysis of the effects of cocaine on the enhancement of HIV-1 replication in normal human astrocytes (NHA)

Jessica L. Reynolds; Supriya D. Mahajan; B. Bindukumar; Donald Sykes; Stanley A. Schwartz; Madhavan P.N. Nair

doi:10.1016/j.brainres.2006.09.034

Proteomic analysis of the effects of cocaine on the enhancement of HIV-1 replication in normal human astrocytes (NHA)

Jessica L. Reynolds
, Supriya D. Mahajan
, B. Bindukumar
, Donald Sykes
, Stanley A. Schwartz
, Madhavan P.N. Nair

Medicine

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

The US is experiencing an epidemic of cocaine use entangled with HIV-1 infection. Normal human astrocytes (NHA) are susceptible to HIV-1 infection. We utilized LTR-R/U5 amplification, p24 antigen assay and the proteomic method of difference gel electrophoresis (DIGE) combined with protein identification through HPLC-MS/MS to investigate the effect of cocaine on HIV-1 infectivity and the proteomic profile of NHA, respectively. Data demonstrate that cocaine significantly upregulates HIV-1 infection in NHA as measured by LTR-R/U5 amplification and p24 antigen assay. Further, our results show for the first time that cocaine differentially regulates the expression of a number of proteins by NHA that may play a role in the neuropathogenesis of HIV-1 disease.

Original language	English
Pages (from-to)	226-236
Number of pages	11
Journal	Brain Research
Volume	1123
Issue number	1
DOIs	https://doi.org/10.1016/j.brainres.2006.09.034
State	Published - Dec 6 2006

Keywords

Cocaine
Difference gel electrophoresis (DIGE)
High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
Normal human astrocytes (NHA)

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10.1016/j.brainres.2006.09.034

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abstract = "The US is experiencing an epidemic of cocaine use entangled with HIV-1 infection. Normal human astrocytes (NHA) are susceptible to HIV-1 infection. We utilized LTR-R/U5 amplification, p24 antigen assay and the proteomic method of difference gel electrophoresis (DIGE) combined with protein identification through HPLC-MS/MS to investigate the effect of cocaine on HIV-1 infectivity and the proteomic profile of NHA, respectively. Data demonstrate that cocaine significantly upregulates HIV-1 infection in NHA as measured by LTR-R/U5 amplification and p24 antigen assay. Further, our results show for the first time that cocaine differentially regulates the expression of a number of proteins by NHA that may play a role in the neuropathogenesis of HIV-1 disease.",

keywords = "Cocaine, Difference gel electrophoresis (DIGE), High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), Normal human astrocytes (NHA)",

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AU - Reynolds, Jessica L.

AU - Mahajan, Supriya D.

AU - Bindukumar, B.

AU - Sykes, Donald

AU - Schwartz, Stanley A.

AU - Nair, Madhavan P.N.

PY - 2006/12/6

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AB - The US is experiencing an epidemic of cocaine use entangled with HIV-1 infection. Normal human astrocytes (NHA) are susceptible to HIV-1 infection. We utilized LTR-R/U5 amplification, p24 antigen assay and the proteomic method of difference gel electrophoresis (DIGE) combined with protein identification through HPLC-MS/MS to investigate the effect of cocaine on HIV-1 infectivity and the proteomic profile of NHA, respectively. Data demonstrate that cocaine significantly upregulates HIV-1 infection in NHA as measured by LTR-R/U5 amplification and p24 antigen assay. Further, our results show for the first time that cocaine differentially regulates the expression of a number of proteins by NHA that may play a role in the neuropathogenesis of HIV-1 disease.

KW - Cocaine

KW - Difference gel electrophoresis (DIGE)

KW - High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)

KW - Normal human astrocytes (NHA)

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Proteomic analysis of the effects of cocaine on the enhancement of HIV-1 replication in normal human astrocytes (NHA)

Abstract

Keywords

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