Prostaglandin E2 inhibits phosphoinositde metabolism in isolated pancreatic islets

S. G. Laychock

doi:10.1042/bj2600291

Prostaglandin E₂ inhibits phosphoinositde metabolism in isolated pancreatic islets

S. G. Laychock

Pharmacology and Toxicology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Isolated islets of the rat labelled with myo-[³H]inositol showed decreased accumulation of total inositol phosphates (InsPs) and [³H]polyphosphoinositide hydrolysis in response to glucose after preincubation with prostaglandin E₂ (PGE₂). The response was concentration-dependent and specific for PGE₂. PGE₂ did not affect basal [³H]phosphoinositide hydrolysis or InsPs accumulation. Pertussis-toxin pretreatment antagonized the response to PGE₂, whereas 8-bromo cyclic AMP was without effect. The PGE₂-induced decrease in InsPs may contribute to the suppression of insulin secretion.

Original language	English
Pages (from-to)	291-294
Number of pages	4
Journal	Biochemical Journal
Volume	260
Issue number	1
DOIs	https://doi.org/10.1042/bj2600291
State	Published - 1989

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abstract = "Isolated islets of the rat labelled with myo-[3H]inositol showed decreased accumulation of total inositol phosphates (InsPs) and [3H]polyphosphoinositide hydrolysis in response to glucose after preincubation with prostaglandin E2 (PGE2). The response was concentration-dependent and specific for PGE2. PGE2 did not affect basal [3H]phosphoinositide hydrolysis or InsPs accumulation. Pertussis-toxin pretreatment antagonized the response to PGE2, whereas 8-bromo cyclic AMP was without effect. The PGE2-induced decrease in InsPs may contribute to the suppression of insulin secretion.",

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AU - Laychock, S. G.

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AB - Isolated islets of the rat labelled with myo-[3H]inositol showed decreased accumulation of total inositol phosphates (InsPs) and [3H]polyphosphoinositide hydrolysis in response to glucose after preincubation with prostaglandin E2 (PGE2). The response was concentration-dependent and specific for PGE2. PGE2 did not affect basal [3H]phosphoinositide hydrolysis or InsPs accumulation. Pertussis-toxin pretreatment antagonized the response to PGE2, whereas 8-bromo cyclic AMP was without effect. The PGE2-induced decrease in InsPs may contribute to the suppression of insulin secretion.

UR - https://www.scopus.com/pages/publications/0024473756

U2 - 10.1042/bj2600291

DO - 10.1042/bj2600291

M3 - Article

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SN - 0264-6021

VL - 260

SP - 291

EP - 294

JO - Biochemical Journal

JF - Biochemical Journal

IS - 1

ER -

Prostaglandin E₂ inhibits phosphoinositde metabolism in isolated pancreatic islets

Abstract

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