Prostacyclin: Effects on cyclic AMP in bone cells

J. C. Robin; M. J. Brown; N. Weinfeld; R. Dziak

Prostacyclin: Effects on cyclic AMP in bone cells

J. C. Robin
, M. J. Brown
, N. Weinfeld
, R. Dziak

Department of Oral Biology

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Prostacyclin (PGI₂) increased cyclic AMP in specific rat bone cell populations obtained by sequential collagenase digestion of calvariae. An osteoclastic population, characterized by high acid phosphatase levels and the ability to resorb bone in vitro, was more sensitive as reflected by responses to lower doses of PGI₂ (2.3 x 10^-8M) as well as greater responses to higher doses (3.5 x 10^-4) of PGI₂ than the other populations isolated. The osteoblastic populations, characterized by high alkaline phosphatase levels and the inability to resorb devitalized bone did not respond significantly to PGI₂ at doses less than 10^-4M and increases in cAMP were smaller. Then results suggest a differential effect of PGI₂ on osteoclasts and osteoblasts which may be involved in the mode of action of endogenously produced PGI₂ in bone.

Original language	English
Pages (from-to)	43-49
Number of pages	7
Journal	Research Communications in Chemical Pathology and Pharmacology
Volume	35
Issue number	1
State	Published - 1982

Cite this

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abstract = "Prostacyclin (PGI2) increased cyclic AMP in specific rat bone cell populations obtained by sequential collagenase digestion of calvariae. An osteoclastic population, characterized by high acid phosphatase levels and the ability to resorb bone in vitro, was more sensitive as reflected by responses to lower doses of PGI2 (2.3 x 10-8M) as well as greater responses to higher doses (3.5 x 10-4) of PGI2 than the other populations isolated. The osteoblastic populations, characterized by high alkaline phosphatase levels and the inability to resorb devitalized bone did not respond significantly to PGI2 at doses less than 10-4M and increases in cAMP were smaller. Then results suggest a differential effect of PGI2 on osteoclasts and osteoblasts which may be involved in the mode of action of endogenously produced PGI2 in bone.",

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AU - Dziak, R.

PY - 1982

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N2 - Prostacyclin (PGI2) increased cyclic AMP in specific rat bone cell populations obtained by sequential collagenase digestion of calvariae. An osteoclastic population, characterized by high acid phosphatase levels and the ability to resorb bone in vitro, was more sensitive as reflected by responses to lower doses of PGI2 (2.3 x 10-8M) as well as greater responses to higher doses (3.5 x 10-4) of PGI2 than the other populations isolated. The osteoblastic populations, characterized by high alkaline phosphatase levels and the inability to resorb devitalized bone did not respond significantly to PGI2 at doses less than 10-4M and increases in cAMP were smaller. Then results suggest a differential effect of PGI2 on osteoclasts and osteoblasts which may be involved in the mode of action of endogenously produced PGI2 in bone.

AB - Prostacyclin (PGI2) increased cyclic AMP in specific rat bone cell populations obtained by sequential collagenase digestion of calvariae. An osteoclastic population, characterized by high acid phosphatase levels and the ability to resorb bone in vitro, was more sensitive as reflected by responses to lower doses of PGI2 (2.3 x 10-8M) as well as greater responses to higher doses (3.5 x 10-4) of PGI2 than the other populations isolated. The osteoblastic populations, characterized by high alkaline phosphatase levels and the inability to resorb devitalized bone did not respond significantly to PGI2 at doses less than 10-4M and increases in cAMP were smaller. Then results suggest a differential effect of PGI2 on osteoclasts and osteoblasts which may be involved in the mode of action of endogenously produced PGI2 in bone.

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AN - SCOPUS:0020040102

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JO - Research Communications in Chemical Pathology and Pharmacology

JF - Research Communications in Chemical Pathology and Pharmacology

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Prostacyclin: Effects on cyclic AMP in bone cells

Abstract

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