Probing the Dynamics of Surface-Immobilized Bioreceptors Using Picosecond Time-Resolved Spectroscopy

Frank V. Bright; Run Wang; Ming Li; Richard A. Dunbar

doi:10.1006/immu.1993.1044

Probing the Dynamics of Surface-Immobilized Bioreceptors Using Picosecond Time-Resolved Spectroscopy

Frank V. Bright
, Run Wang
, Ming Li
, Richard A. Dunbar

Chemistry

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Total internal reflection fluorescence (TIRF) is used in concert with the multifrequency phase and modulation (MPM) technique to study in situ the interfacial distribution of surface-immobilized F(ab′) antifluorescein antibodies. Using this new technique we recover information on the surface-immobilized receptor dynamics and demonstrate that this particular immunosurface is composed of an unstable distribution of biodomains. The MPM-TIRF technique adds a new tool to our arsenal for studying the dynamics of biosensor interfaces.

Original language	English
Pages (from-to)	104-111
Number of pages	8
Journal	ImmunoMethods
Volume	3
Issue number	2
DOIs	https://doi.org/10.1006/immu.1993.1044
State	Published - Oct 1993

Access to Document

10.1006/immu.1993.1044

Cite this

@article{ed69b692100a4424ac2d3db93fecb0dc,

title = "Probing the Dynamics of Surface-Immobilized Bioreceptors Using Picosecond Time-Resolved Spectroscopy",

abstract = "Total internal reflection fluorescence (TIRF) is used in concert with the multifrequency phase and modulation (MPM) technique to study in situ the interfacial distribution of surface-immobilized F(ab′) antifluorescein antibodies. Using this new technique we recover information on the surface-immobilized receptor dynamics and demonstrate that this particular immunosurface is composed of an unstable distribution of biodomains. The MPM-TIRF technique adds a new tool to our arsenal for studying the dynamics of biosensor interfaces.",

author = "Bright, \{Frank V.\} and Run Wang and Ming Li and Dunbar, \{Richard A.\}",

year = "1993",

month = oct,

doi = "10.1006/immu.1993.1044",

language = "English",

volume = "3",

pages = "104--111",

journal = "ImmunoMethods",

issn = "1058-6687",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Probing the Dynamics of Surface-Immobilized Bioreceptors Using Picosecond Time-Resolved Spectroscopy

AU - Bright, Frank V.

AU - Wang, Run

AU - Li, Ming

AU - Dunbar, Richard A.

PY - 1993/10

Y1 - 1993/10

N2 - Total internal reflection fluorescence (TIRF) is used in concert with the multifrequency phase and modulation (MPM) technique to study in situ the interfacial distribution of surface-immobilized F(ab′) antifluorescein antibodies. Using this new technique we recover information on the surface-immobilized receptor dynamics and demonstrate that this particular immunosurface is composed of an unstable distribution of biodomains. The MPM-TIRF technique adds a new tool to our arsenal for studying the dynamics of biosensor interfaces.

AB - Total internal reflection fluorescence (TIRF) is used in concert with the multifrequency phase and modulation (MPM) technique to study in situ the interfacial distribution of surface-immobilized F(ab′) antifluorescein antibodies. Using this new technique we recover information on the surface-immobilized receptor dynamics and demonstrate that this particular immunosurface is composed of an unstable distribution of biodomains. The MPM-TIRF technique adds a new tool to our arsenal for studying the dynamics of biosensor interfaces.

UR - https://www.scopus.com/pages/publications/0344689183

U2 - 10.1006/immu.1993.1044

DO - 10.1006/immu.1993.1044

M3 - Article

AN - SCOPUS:0344689183

SN - 1058-6687

VL - 3

SP - 104

EP - 111

JO - ImmunoMethods

JF - ImmunoMethods

IS - 2

ER -

Probing the Dynamics of Surface-Immobilized Bioreceptors Using Picosecond Time-Resolved Spectroscopy

Abstract

Access to Document

Other files and links

Fingerprint

Cite this