Abstract
A method is described for culturing primary rabbit kidney epithelial cells which express proximal tubule functions. First, rabbit kidney proximal tubules are purified in a sterile manner. Then the tubules are put into serum free culture medium supplemented with insulin, transferrin and hydrocortisone. After one week, confluent monolayers of epithelial cells are obtained which can be used for investigations of interest.
| Original language | English |
|---|---|
| Pages (from-to) | 67-71 |
| Number of pages | 5 |
| Journal | Journal of Tissue Culture Methods |
| Volume | 9 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 1985 |
Keywords
- defined medium
- kidney cells
- primary culture
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