Presence of a bent helix in fragments of kinetoplast DNA minicircles from several trypanosomatid species

James M. Ntambi; Joan C. Marini; James D. Bangs; Stephen L. Hajduk; Hector E. Jimenez; Peter A. Kitchin; Viiu A. Klein; Kathleen A. Ryan; Paul T. Englund

doi:10.1016/0166-6851(84)90084-7

Presence of a bent helix in fragments of kinetoplast DNA minicircles from several trypanosomatid species

James M. Ntambi
, Joan C. Marini
, James D. Bangs
, Stephen L. Hajduk
, Hector E. Jimenez
, Peter A. Kitchin
, Viiu A. Klein
, Kathleen A. Ryan
, Paul T. Englund

Microbiology and Immunology

Johns Hopkins University

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Some restriction fragments of kinetoplast minicircles from several trypanosomatid species (Leishmania tarentolae, Trypanosoma brucei, T. equiperdum, Herpetomonas muscarum, Crithidia fasciculata, but not T. cruzi) migrate anomalously on polyacrylamide gels. This behavior is probably due to a natural curvature of the helix. Bent helices appear to be a common property of kinetoplast minicircles, and may be important for minicircle function. In the case of T. equiperdum, we present evidence that each minicircle has a single bent region which resides in or near the 'conserved sequence'.

Original language	English
Pages (from-to)	273-286
Number of pages	14
Journal	Molecular and Biochemical Parasitology
Volume	12
Issue number	3
DOIs	https://doi.org/10.1016/0166-6851(84)90084-7
State	Published - Jul 1984

Keywords

Bent helix
Kinetoplast DNA
Minicircle
Trypanosomatid

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10.1016/0166-6851(84)90084-7

Cite this

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title = "Presence of a bent helix in fragments of kinetoplast DNA minicircles from several trypanosomatid species",

abstract = "Some restriction fragments of kinetoplast minicircles from several trypanosomatid species (Leishmania tarentolae, Trypanosoma brucei, T. equiperdum, Herpetomonas muscarum, Crithidia fasciculata, but not T. cruzi) migrate anomalously on polyacrylamide gels. This behavior is probably due to a natural curvature of the helix. Bent helices appear to be a common property of kinetoplast minicircles, and may be important for minicircle function. In the case of T. equiperdum, we present evidence that each minicircle has a single bent region which resides in or near the 'conserved sequence'.",

keywords = "Bent helix, Kinetoplast DNA, Minicircle, Trypanosomatid",

author = "Ntambi, \{James M.\} and Marini, \{Joan C.\} and Bangs, \{James D.\} and Hajduk, \{Stephen L.\} and Jimenez, \{Hector E.\} and Kitchin, \{Peter A.\} and Klein, \{Viiu A.\} and Ryan, \{Kathleen A.\} and Englund, \{Paul T.\}",

year = "1984",

month = jul,

doi = "10.1016/0166-6851(84)90084-7",

language = "English",

volume = "12",

pages = "273--286",

journal = "Molecular and Biochemical Parasitology",

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publisher = "Elsevier B.V.",

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}

TY - JOUR

T1 - Presence of a bent helix in fragments of kinetoplast DNA minicircles from several trypanosomatid species

AU - Ntambi, James M.

AU - Marini, Joan C.

AU - Bangs, James D.

AU - Hajduk, Stephen L.

AU - Jimenez, Hector E.

AU - Kitchin, Peter A.

AU - Klein, Viiu A.

AU - Ryan, Kathleen A.

AU - Englund, Paul T.

PY - 1984/7

Y1 - 1984/7

N2 - Some restriction fragments of kinetoplast minicircles from several trypanosomatid species (Leishmania tarentolae, Trypanosoma brucei, T. equiperdum, Herpetomonas muscarum, Crithidia fasciculata, but not T. cruzi) migrate anomalously on polyacrylamide gels. This behavior is probably due to a natural curvature of the helix. Bent helices appear to be a common property of kinetoplast minicircles, and may be important for minicircle function. In the case of T. equiperdum, we present evidence that each minicircle has a single bent region which resides in or near the 'conserved sequence'.

AB - Some restriction fragments of kinetoplast minicircles from several trypanosomatid species (Leishmania tarentolae, Trypanosoma brucei, T. equiperdum, Herpetomonas muscarum, Crithidia fasciculata, but not T. cruzi) migrate anomalously on polyacrylamide gels. This behavior is probably due to a natural curvature of the helix. Bent helices appear to be a common property of kinetoplast minicircles, and may be important for minicircle function. In the case of T. equiperdum, we present evidence that each minicircle has a single bent region which resides in or near the 'conserved sequence'.

KW - Bent helix

KW - Kinetoplast DNA

KW - Minicircle

KW - Trypanosomatid

UR - https://www.scopus.com/pages/publications/0021186637

U2 - 10.1016/0166-6851(84)90084-7

DO - 10.1016/0166-6851(84)90084-7

M3 - Article

C2 - 6090900

AN - SCOPUS:0021186637

SN - 0166-6851

VL - 12

SP - 273

EP - 286

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

IS - 3

ER -

Presence of a bent helix in fragments of kinetoplast DNA minicircles from several trypanosomatid species

Abstract

Keywords

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