Precursor-directed polyketide biosynthesis in Escherichia coli

Kenji Kinoshita; Blaine A. Pfeifer; Chaitan Khosla; David E. Cane

doi:10.1016/j.bmcl.2003.08.008

Precursor-directed polyketide biosynthesis in Escherichia coli

Kenji Kinoshita
, Blaine A. Pfeifer
, Chaitan Khosla
, David E. Cane

Department of Chemical and Biological Engineering

Brown University
Stanford University

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Precursor-directed polyketide biosynthesis was demonstrated in the heterologous host Escherichia coli. Several diketide and triketide substrates were fed to a recombinant E. coli strain containing a variant form of deoxyerythronolide B synthase (DEBS) from which the first elongation module was deleted resulting in successful macrolactone formation from the diketide, but not the triketide, substrates.

Original language	English
Pages (from-to)	3701-3704
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	13
Issue number	21
DOIs	https://doi.org/10.1016/j.bmcl.2003.08.008
State	Published - Nov 3 2003

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10.1016/j.bmcl.2003.08.008

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title = "Precursor-directed polyketide biosynthesis in Escherichia coli",

abstract = "Precursor-directed polyketide biosynthesis was demonstrated in the heterologous host Escherichia coli. Several diketide and triketide substrates were fed to a recombinant E. coli strain containing a variant form of deoxyerythronolide B synthase (DEBS) from which the first elongation module was deleted resulting in successful macrolactone formation from the diketide, but not the triketide, substrates.",

author = "Kenji Kinoshita and Pfeifer, \{Blaine A.\} and Chaitan Khosla and Cane, \{David E.\}",

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AU - Kinoshita, Kenji

AU - Pfeifer, Blaine A.

AU - Khosla, Chaitan

AU - Cane, David E.

PY - 2003/11/3

Y1 - 2003/11/3

N2 - Precursor-directed polyketide biosynthesis was demonstrated in the heterologous host Escherichia coli. Several diketide and triketide substrates were fed to a recombinant E. coli strain containing a variant form of deoxyerythronolide B synthase (DEBS) from which the first elongation module was deleted resulting in successful macrolactone formation from the diketide, but not the triketide, substrates.

AB - Precursor-directed polyketide biosynthesis was demonstrated in the heterologous host Escherichia coli. Several diketide and triketide substrates were fed to a recombinant E. coli strain containing a variant form of deoxyerythronolide B synthase (DEBS) from which the first elongation module was deleted resulting in successful macrolactone formation from the diketide, but not the triketide, substrates.

UR - https://www.scopus.com/pages/publications/0141656418

U2 - 10.1016/j.bmcl.2003.08.008

DO - 10.1016/j.bmcl.2003.08.008

M3 - Article

C2 - 14552761

AN - SCOPUS:0141656418

SN - 0960-894X

VL - 13

SP - 3701

EP - 3704

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 21

ER -

Precursor-directed polyketide biosynthesis in Escherichia coli

Abstract

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