Photobleaching under single photon and multi-photon excitation: Chloroplasts in protoplasts from Arabidopsis thaliana

Fu Jen Kao; Yi Min Wang; Jian Cheng Chen; Ping Chin Cheng; Rung Wu Chen; Bai Ling Lin

doi:10.1016/S0030-4018(01)01679-0

Photobleaching under single photon and multi-photon excitation: Chloroplasts in protoplasts from Arabidopsis thaliana

Fu Jen Kao
, Yi Min Wang
, Jian Cheng Chen
, Ping Chin Cheng
, Rung Wu Chen
, Bai Ling Lin

Department of Electrical Engineering

National Sun Yat-sen University
Academia Sinica - Institute of Molecular Biology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Photobleaching as a result of intense laser irradiation has been an important issue in the applications of confocal microscopy. In this study we are employing time-lapsed micro-spectroscopy to compare the effects of photobleaching under single photon and multi-photon excitation. A model is derived to account for photobleaching in both cases and protoplasts from Arabidopsis thaliana are employed as the sample. We have found that multi-photon excitation have impacts on the samples in ways much different from cw illumination in the linear domain.

Original language	English
Pages (from-to)	85-91
Number of pages	7
Journal	Optics Communications
Volume	201
Issue number	1-3
DOIs	https://doi.org/10.1016/S0030-4018(01)01679-0
State	Published - Jan 1 2002

Keywords

Chloroplasts
Micro-spectroscopy
Multi-phpton excition
Photobleaching

Access to Document

10.1016/S0030-4018(01)01679-0

Cite this

@article{13571c33c1c24ad4bfb87512c32befc9,

title = "Photobleaching under single photon and multi-photon excitation: Chloroplasts in protoplasts from Arabidopsis thaliana",

abstract = "Photobleaching as a result of intense laser irradiation has been an important issue in the applications of confocal microscopy. In this study we are employing time-lapsed micro-spectroscopy to compare the effects of photobleaching under single photon and multi-photon excitation. A model is derived to account for photobleaching in both cases and protoplasts from Arabidopsis thaliana are employed as the sample. We have found that multi-photon excitation have impacts on the samples in ways much different from cw illumination in the linear domain.",

keywords = "Chloroplasts, Micro-spectroscopy, Multi-phpton excition, Photobleaching",

author = "Kao, \{Fu Jen\} and Wang, \{Yi Min\} and Chen, \{Jian Cheng\} and Cheng, \{Ping Chin\} and Chen, \{Rung Wu\} and Lin, \{Bai Ling\}",

year = "2002",

month = jan,

day = "1",

doi = "10.1016/S0030-4018(01)01679-0",

language = "English",

volume = "201",

pages = "85--91",

journal = "Optics Communications",

issn = "0030-4018",

publisher = "Elsevier B.V.",

number = "1-3",

}

TY - JOUR

T1 - Photobleaching under single photon and multi-photon excitation

T2 - Chloroplasts in protoplasts from Arabidopsis thaliana

AU - Kao, Fu Jen

AU - Wang, Yi Min

AU - Chen, Jian Cheng

AU - Cheng, Ping Chin

AU - Chen, Rung Wu

AU - Lin, Bai Ling

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Photobleaching as a result of intense laser irradiation has been an important issue in the applications of confocal microscopy. In this study we are employing time-lapsed micro-spectroscopy to compare the effects of photobleaching under single photon and multi-photon excitation. A model is derived to account for photobleaching in both cases and protoplasts from Arabidopsis thaliana are employed as the sample. We have found that multi-photon excitation have impacts on the samples in ways much different from cw illumination in the linear domain.

AB - Photobleaching as a result of intense laser irradiation has been an important issue in the applications of confocal microscopy. In this study we are employing time-lapsed micro-spectroscopy to compare the effects of photobleaching under single photon and multi-photon excitation. A model is derived to account for photobleaching in both cases and protoplasts from Arabidopsis thaliana are employed as the sample. We have found that multi-photon excitation have impacts on the samples in ways much different from cw illumination in the linear domain.

KW - Chloroplasts

KW - Micro-spectroscopy

KW - Multi-phpton excition

KW - Photobleaching

UR - https://www.scopus.com/pages/publications/0036139350

U2 - 10.1016/S0030-4018(01)01679-0

DO - 10.1016/S0030-4018(01)01679-0

M3 - Article

AN - SCOPUS:0036139350

SN - 0030-4018

VL - 201

SP - 85

EP - 91

JO - Optics Communications

JF - Optics Communications

IS - 1-3

ER -

Photobleaching under single photon and multi-photon excitation: Chloroplasts in protoplasts from Arabidopsis thaliana

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this