@inproceedings{58327657a6814b9698cebe61e68ee01e,
title = "Photoacoustic imaging of the near-infrared fluorescent protein iRFP in vivo",
abstract = "Genetically encoded probes powerfully and non-invasively target specific tissues, cells, and subcellular locations. iRFP, a novel near-infrared fluorescent protein with low quantum yield whose absorption and fluorescence maxima are located at wavelengths longer than the Q-band of hemoglobin absorption, is ideal for PAT. Here, we report on an in vitro comparison of iRFP with other far-red fluorescent proteins, and its use in imaging a mouse tumor xenograft model. In an in vivo experiment, we stably transfected iRFP into MTLn3 adenocarcinoma cells and injected them into the mammary fat pad of female SCID/NCr mice, then imaged the resulting tumors two and three weeks post injection. The contrast increase from the protein expression was high enough to clearly separate the tumor region from the rest of the animal.",
keywords = "Deep imaging, Fluorescent proteins, Genetically encoded probe, IRFP, Photoacoustic tomography",
author = "Arie Krumholz and Filonov, \{Grigory S.\} and Jun Xia and Junjie Yao and Verkhusha, \{Vladislav V.\} and Wang, \{Lihong V.\}",
year = "2012",
month = feb,
day = "9",
doi = "10.1117/12.908927",
language = "English",
isbn = "9780819488664",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
booktitle = "Photons Plus Ultrasound",
address = "United States",
note = "Photons Plus Ultrasound: Imaging and Sensing 2012 ; Conference date: 22-01-2012 Through 24-01-2012",
}