Phase fluorometric glucose biosensor using oxygen as transducer and enzyme-doped xerogels

R. M. Bukowski; V. P. Chodavarapu; A. H. Titus; A. N. Cartwright; F. V. Bright

doi:10.1049/el:20070078

Phase fluorometric glucose biosensor using oxygen as transducer and enzyme-doped xerogels

R. M. Bukowski
, V. P. Chodavarapu
, A. H. Titus
, A. N. Cartwright
, F. V. Bright

SUNY Buffalo
McGill University

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The development of a glucose biosensor based on co-immobilisation of oxygen (O₂) responsive luminophore and glucose oxidase (GOx) within nanoporous solgel-derived xerogels is described. The biosensor operates in the frequency domain and exploits the effect of glucose and O₂ consumption by GOx on the luminophore excited-state lifetime (i.e. phase angle). The biosensor consists of a modulated light emitting diode as the excitation source and a silicon photodiode as the detector. This sensing methodology establishes the viability for low-cost and accurate biosensors that operate at a low modulation frequency (tens of kHz). The biosensor is stable, reproducible, and provides an analytically reliable response from 0.5 to 15mM glucose.

Original language	English
Pages (from-to)	202-204
Number of pages	3
Journal	Electronics Letters
Volume	43
Issue number	4
DOIs	https://doi.org/10.1049/el:20070078
State	Published - 2007

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10.1049/el:20070078

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title = "Phase fluorometric glucose biosensor using oxygen as transducer and enzyme-doped xerogels",

abstract = "The development of a glucose biosensor based on co-immobilisation of oxygen (O2) responsive luminophore and glucose oxidase (GOx) within nanoporous solgel-derived xerogels is described. The biosensor operates in the frequency domain and exploits the effect of glucose and O2 consumption by GOx on the luminophore excited-state lifetime (i.e. phase angle). The biosensor consists of a modulated light emitting diode as the excitation source and a silicon photodiode as the detector. This sensing methodology establishes the viability for low-cost and accurate biosensors that operate at a low modulation frequency (tens of kHz). The biosensor is stable, reproducible, and provides an analytically reliable response from 0.5 to 15mM glucose.",

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year = "2007",

doi = "10.1049/el:20070078",

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pages = "202--204",

journal = "Electronics Letters",

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T1 - Phase fluorometric glucose biosensor using oxygen as transducer and enzyme-doped xerogels

AU - Bukowski, R. M.

AU - Chodavarapu, V. P.

AU - Titus, A. H.

AU - Cartwright, A. N.

AU - Bright, F. V.

PY - 2007

Y1 - 2007

N2 - The development of a glucose biosensor based on co-immobilisation of oxygen (O2) responsive luminophore and glucose oxidase (GOx) within nanoporous solgel-derived xerogels is described. The biosensor operates in the frequency domain and exploits the effect of glucose and O2 consumption by GOx on the luminophore excited-state lifetime (i.e. phase angle). The biosensor consists of a modulated light emitting diode as the excitation source and a silicon photodiode as the detector. This sensing methodology establishes the viability for low-cost and accurate biosensors that operate at a low modulation frequency (tens of kHz). The biosensor is stable, reproducible, and provides an analytically reliable response from 0.5 to 15mM glucose.

AB - The development of a glucose biosensor based on co-immobilisation of oxygen (O2) responsive luminophore and glucose oxidase (GOx) within nanoporous solgel-derived xerogels is described. The biosensor operates in the frequency domain and exploits the effect of glucose and O2 consumption by GOx on the luminophore excited-state lifetime (i.e. phase angle). The biosensor consists of a modulated light emitting diode as the excitation source and a silicon photodiode as the detector. This sensing methodology establishes the viability for low-cost and accurate biosensors that operate at a low modulation frequency (tens of kHz). The biosensor is stable, reproducible, and provides an analytically reliable response from 0.5 to 15mM glucose.

UR - https://www.scopus.com/pages/publications/33847123576

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DO - 10.1049/el:20070078

M3 - Article

AN - SCOPUS:33847123576

SN - 0013-5194

VL - 43

SP - 202

EP - 204

JO - Electronics Letters

JF - Electronics Letters

IS - 4

ER -

Phase fluorometric glucose biosensor using oxygen as transducer and enzyme-doped xerogels

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