Pesticides in sediments from Magdalena River, Colombia, are linked to reproductive toxicity on Caenorhabditis elegans

Pesticides are prevalent pollutants found in river sediments in agricultural regions worldwide, leading to environmental pollution and toxic effects on biota. In this study, twenty sediment samples were collected from the Magdalena River in Colombia and analyzed for forty pesticides. Methanolic extracts of the sediments were used to expose Caenorhabditis elegans for 24 h, evaluating the effects on its reproduction. The most abundant pesticides found in Magdalena River sediments were atrazine, bromacil, DDE, and chlorpyrifos. The concentrations of DDE and the sum of DDD, DDE, and DDT were above the Threshold Effect Concentration (TEC) values for freshwater sediments, indicating potential effects on aquatic organisms. The ratios of DDT/(DDE + DDD) and DDD/DDE suggest historical contributions of DDT and degradation under aerobic conditions. Several sampling sites displayed a moderate toxicity risk to biota, as calculated by the sediment quality guideline quotient (SQGQ). Nematode brood size was reduced by up to 37% after sediment extract exposure. The presence of chlordane, DDT-related compounds, and chlorpyrifos in Magdalena River sediments was associated with reproductive toxicity among C. elegans.