Pertussis toxin inhibits chemotactic factor-induced phospholipase C stimulation and lysosomal enzyme secretion in rabbit neutrophils

Peter G. Bradford; Ronald P. Rubin

doi:10.1016/0014-5793(85)80801-2

Pertussis toxin inhibits chemotactic factor-induced phospholipase C stimulation and lysosomal enzyme secretion in rabbit neutrophils

Peter G. Bradford
, Ronald P. Rubin

Pharmacology and Toxicology

Virginia Commonwealth University

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Pertussis toxin suppressed [³²P]polyphosphoinositide breakdown and lysosomal enzyme secretion induced by fMet-Leu-Phe in rabbit neutrophils. Likewise, fMet-Leu-Phe- or leukotriene B4-evoked (³H]inositol trisphosphate accumulation was inhibited by the toxin. These findings, taken together with evidence that pertussis toxin specifically causes inactivation of the guanine nucleotide binding protein (N_i), suggests that guanine nucleotide binding proteins may mediate coupling between calcium-mobilising receptors and phospholipase C-mediated reactions in rabbit neutrophils.

Original language	English
Pages (from-to)	317-320
Number of pages	4
Journal	FEBS Letters
Volume	183
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(85)80801-2
State	Published - Apr 22 1985

Keywords

Inositol trisphosphate
Lysosomal enzyme secretion
Pertussis toxin
Phospholipase C
Rabbit neutrophil

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10.1016/0014-5793(85)80801-2

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title = "Pertussis toxin inhibits chemotactic factor-induced phospholipase C stimulation and lysosomal enzyme secretion in rabbit neutrophils",

abstract = "Pertussis toxin suppressed [32P]polyphosphoinositide breakdown and lysosomal enzyme secretion induced by fMet-Leu-Phe in rabbit neutrophils. Likewise, fMet-Leu-Phe- or leukotriene B4-evoked (3H]inositol trisphosphate accumulation was inhibited by the toxin. These findings, taken together with evidence that pertussis toxin specifically causes inactivation of the guanine nucleotide binding protein (Ni), suggests that guanine nucleotide binding proteins may mediate coupling between calcium-mobilising receptors and phospholipase C-mediated reactions in rabbit neutrophils.",

keywords = "Inositol trisphosphate, Lysosomal enzyme secretion, Pertussis toxin, Phospholipase C, Rabbit neutrophil",

author = "Bradford, \{Peter G.\} and Rubin, \{Ronald P.\}",

year = "1985",

month = apr,

day = "22",

doi = "10.1016/0014-5793(85)80801-2",

language = "English",

volume = "183",

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T1 - Pertussis toxin inhibits chemotactic factor-induced phospholipase C stimulation and lysosomal enzyme secretion in rabbit neutrophils

AU - Bradford, Peter G.

AU - Rubin, Ronald P.

PY - 1985/4/22

Y1 - 1985/4/22

N2 - Pertussis toxin suppressed [32P]polyphosphoinositide breakdown and lysosomal enzyme secretion induced by fMet-Leu-Phe in rabbit neutrophils. Likewise, fMet-Leu-Phe- or leukotriene B4-evoked (3H]inositol trisphosphate accumulation was inhibited by the toxin. These findings, taken together with evidence that pertussis toxin specifically causes inactivation of the guanine nucleotide binding protein (Ni), suggests that guanine nucleotide binding proteins may mediate coupling between calcium-mobilising receptors and phospholipase C-mediated reactions in rabbit neutrophils.

AB - Pertussis toxin suppressed [32P]polyphosphoinositide breakdown and lysosomal enzyme secretion induced by fMet-Leu-Phe in rabbit neutrophils. Likewise, fMet-Leu-Phe- or leukotriene B4-evoked (3H]inositol trisphosphate accumulation was inhibited by the toxin. These findings, taken together with evidence that pertussis toxin specifically causes inactivation of the guanine nucleotide binding protein (Ni), suggests that guanine nucleotide binding proteins may mediate coupling between calcium-mobilising receptors and phospholipase C-mediated reactions in rabbit neutrophils.

KW - Inositol trisphosphate

KW - Lysosomal enzyme secretion

KW - Pertussis toxin

KW - Phospholipase C

KW - Rabbit neutrophil

UR - https://www.scopus.com/pages/publications/0021849945

U2 - 10.1016/0014-5793(85)80801-2

DO - 10.1016/0014-5793(85)80801-2

M3 - Article

C2 - 2985432

AN - SCOPUS:0021849945

SN - 0014-5793

VL - 183

SP - 317

EP - 320

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Pertussis toxin inhibits chemotactic factor-induced phospholipase C stimulation and lysosomal enzyme secretion in rabbit neutrophils

Abstract

Keywords

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