Partial purification and characterization of the bone resorption factor from Actinomyces viscosus

E. Hausmann; B. C. Nair; K. W. Knox; K. W. Broady; A. J. Wicken; M. Brown; R. Dziak

doi:10.1007/BF02411208

Partial purification and characterization of the bone resorption factor from Actinomyces viscosus

E. Hausmann
, B. C. Nair
, K. W. Knox
, K. W. Broady
, A. J. Wicken
, M. Brown
, R. Dziak

Department of Oral Biology

SUNY Buffalo
Institute of Dental Research, United Dental Hospital of Sydney
University of New South Wales

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The bone resorptive factor and amphipathic antigen (AcA) previously identified by us in preparations from Actinomyces viscosus have been partially purified, characterized chemically, and compared. They elute at the same location on chromatography with Ac 22. The fatty acid composition of AcA and the bone resorptive factor is the same. Some differences in carbohydrate composition are observed. The Actinomyces factor does not affect calcium influx or cyclic AMP in isolated bone cells. Therefore it is concluded that AcA stimulates resorption either by gaining entrance into bone cells or by way of a yet undetermined second messenger.

Original language	English
Pages (from-to)	49-53
Number of pages	5
Journal	Calcified Tissue International
Volume	34
Issue number	1
DOIs	https://doi.org/10.1007/BF02411208
State	Published - Dec 1982

Keywords

Bacterial amphophile
Ca influx
Chemistry
Cyclic AMP
Purification
Resorption

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10.1007/BF02411208

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abstract = "The bone resorptive factor and amphipathic antigen (AcA) previously identified by us in preparations from Actinomyces viscosus have been partially purified, characterized chemically, and compared. They elute at the same location on chromatography with Ac 22. The fatty acid composition of AcA and the bone resorptive factor is the same. Some differences in carbohydrate composition are observed. The Actinomyces factor does not affect calcium influx or cyclic AMP in isolated bone cells. Therefore it is concluded that AcA stimulates resorption either by gaining entrance into bone cells or by way of a yet undetermined second messenger.",

keywords = "Bacterial amphophile, Ca influx, Chemistry, Cyclic AMP, Purification, Resorption",

author = "E. Hausmann and Nair, \{B. C.\} and Knox, \{K. W.\} and Broady, \{K. W.\} and Wicken, \{A. J.\} and M. Brown and R. Dziak",

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AU - Hausmann, E.

AU - Nair, B. C.

AU - Knox, K. W.

AU - Broady, K. W.

AU - Wicken, A. J.

AU - Brown, M.

AU - Dziak, R.

PY - 1982/12

Y1 - 1982/12

N2 - The bone resorptive factor and amphipathic antigen (AcA) previously identified by us in preparations from Actinomyces viscosus have been partially purified, characterized chemically, and compared. They elute at the same location on chromatography with Ac 22. The fatty acid composition of AcA and the bone resorptive factor is the same. Some differences in carbohydrate composition are observed. The Actinomyces factor does not affect calcium influx or cyclic AMP in isolated bone cells. Therefore it is concluded that AcA stimulates resorption either by gaining entrance into bone cells or by way of a yet undetermined second messenger.

AB - The bone resorptive factor and amphipathic antigen (AcA) previously identified by us in preparations from Actinomyces viscosus have been partially purified, characterized chemically, and compared. They elute at the same location on chromatography with Ac 22. The fatty acid composition of AcA and the bone resorptive factor is the same. Some differences in carbohydrate composition are observed. The Actinomyces factor does not affect calcium influx or cyclic AMP in isolated bone cells. Therefore it is concluded that AcA stimulates resorption either by gaining entrance into bone cells or by way of a yet undetermined second messenger.

KW - Bacterial amphophile

KW - Ca influx

KW - Chemistry

KW - Cyclic AMP

KW - Purification

KW - Resorption

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EP - 53

JO - Calcified Tissue International

JF - Calcified Tissue International

IS - 1

ER -

Partial purification and characterization of the bone resorption factor from Actinomyces viscosus

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Keywords

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