Parathyromimetic effects of the ionophore, A23187, on bone cells and organ cultures

R. Dziak; P. Stern

doi:10.1016/S0006-291X(75)80377-9

Parathyromimetic effects of the ionophore, A23187, on bone cells and organ cultures

R. Dziak
, P. Stern

Department of Oral Biology

Northwestern University

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

The ionophore, A23187, increased calcium uptake by isolated bone cells in a manner similar to parathyroid hormone. Cellular cyclic AMP was increased after a 30 minute incubation with 0.3 μg/ml A23187; at higher ionophore concentrations (1.0 - 10.0 μg/ml) cyclic AMP was decreased. Resorption of cultured fetal rat long bone also exhibited a biphasic response to the ionophore. With doses of 0.1 - 1.0 μg/ml resorption was increased; higher doses decreased resorption. The data suggest that calcium may be the primary initiator of the action of parathyroid hormone on bone.

Original language	English
Pages (from-to)	1343-1349
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	65
Issue number	4
DOIs	https://doi.org/10.1016/S0006-291X(75)80377-9
State	Published - Aug 18 1975

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title = "Parathyromimetic effects of the ionophore, A23187, on bone cells and organ cultures",

abstract = "The ionophore, A23187, increased calcium uptake by isolated bone cells in a manner similar to parathyroid hormone. Cellular cyclic AMP was increased after a 30 minute incubation with 0.3 μg/ml A23187; at higher ionophore concentrations (1.0 - 10.0 μg/ml) cyclic AMP was decreased. Resorption of cultured fetal rat long bone also exhibited a biphasic response to the ionophore. With doses of 0.1 - 1.0 μg/ml resorption was increased; higher doses decreased resorption. The data suggest that calcium may be the primary initiator of the action of parathyroid hormone on bone.",

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T1 - Parathyromimetic effects of the ionophore, A23187, on bone cells and organ cultures

AU - Dziak, R.

AU - Stern, P.

PY - 1975/8/18

Y1 - 1975/8/18

N2 - The ionophore, A23187, increased calcium uptake by isolated bone cells in a manner similar to parathyroid hormone. Cellular cyclic AMP was increased after a 30 minute incubation with 0.3 μg/ml A23187; at higher ionophore concentrations (1.0 - 10.0 μg/ml) cyclic AMP was decreased. Resorption of cultured fetal rat long bone also exhibited a biphasic response to the ionophore. With doses of 0.1 - 1.0 μg/ml resorption was increased; higher doses decreased resorption. The data suggest that calcium may be the primary initiator of the action of parathyroid hormone on bone.

AB - The ionophore, A23187, increased calcium uptake by isolated bone cells in a manner similar to parathyroid hormone. Cellular cyclic AMP was increased after a 30 minute incubation with 0.3 μg/ml A23187; at higher ionophore concentrations (1.0 - 10.0 μg/ml) cyclic AMP was decreased. Resorption of cultured fetal rat long bone also exhibited a biphasic response to the ionophore. With doses of 0.1 - 1.0 μg/ml resorption was increased; higher doses decreased resorption. The data suggest that calcium may be the primary initiator of the action of parathyroid hormone on bone.

UR - https://www.scopus.com/pages/publications/0016751673

U2 - 10.1016/S0006-291X(75)80377-9

DO - 10.1016/S0006-291X(75)80377-9

M3 - Article

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AN - SCOPUS:0016751673

SN - 0006-291X

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Parathyromimetic effects of the ionophore, A23187, on bone cells and organ cultures

Abstract

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