One minute ultraviolet exposure inhibits Toxoplasma gondii tachyzoite replication and cyst conversion without diminishing host humoral-mediated immune response

Geetha Kannan; Emese Prandovszky; Curtis B. Steinfeldt; Kristin L. Gressitt; Chun Xia Yang; Robert H. Yolken; Emily G. Severance; Lorraine Jones-Brando; Mikhail V. Pletnikov

doi:10.1016/j.exppara.2014.08.001

One minute ultraviolet exposure inhibits Toxoplasma gondii tachyzoite replication and cyst conversion without diminishing host humoral-mediated immune response

Geetha Kannan
, Emese Prandovszky
, Curtis B. Steinfeldt
, Kristin L. Gressitt
, Chun Xia Yang
, Robert H. Yolken
, Emily G. Severance
, Lorraine Jones-Brando
, Mikhail V. Pletnikov

Physiology and Biophysics

Johns Hopkins University

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We developed a protocol to inactivate Toxoplasma gondii (T. gondii) tachyzoites employing 1. min of ultraviolet (UV) exposure. We show that this treatment completely inhibited parasite replication and cyst formation in vitro and in vivo but did not affect the induction of a robust IgG response in mice. We propose that our protocol can be used to study the contribution of the humoral immune response to rodent behavioral alterations following T. gondii infection.

Original language	English
Pages (from-to)	110-117
Number of pages	8
Journal	Experimental Parasitology
Volume	145
Issue number	1
DOIs	https://doi.org/10.1016/j.exppara.2014.08.001
State	Published - Oct 2014

Keywords

Animal models
Behavior
Cysts
Immune response
Inactivation
Toxoplasma

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10.1016/j.exppara.2014.08.001

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Kannan, G., Prandovszky, E., Steinfeldt, C. B., Gressitt, K. L., Yang, C. X., Yolken, R. H., Severance, E. G., Jones-Brando, L., & Pletnikov, M. V. (2014). One minute ultraviolet exposure inhibits Toxoplasma gondii tachyzoite replication and cyst conversion without diminishing host humoral-mediated immune response. Experimental Parasitology, 145(1), 110-117. https://doi.org/10.1016/j.exppara.2014.08.001

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title = "One minute ultraviolet exposure inhibits Toxoplasma gondii tachyzoite replication and cyst conversion without diminishing host humoral-mediated immune response",

abstract = "We developed a protocol to inactivate Toxoplasma gondii (T. gondii) tachyzoites employing 1. min of ultraviolet (UV) exposure. We show that this treatment completely inhibited parasite replication and cyst formation in vitro and in vivo but did not affect the induction of a robust IgG response in mice. We propose that our protocol can be used to study the contribution of the humoral immune response to rodent behavioral alterations following T. gondii infection.",

keywords = "Animal models, Behavior, Cysts, Immune response, Inactivation, Toxoplasma",

author = "Geetha Kannan and Emese Prandovszky and Steinfeldt, \{Curtis B.\} and Gressitt, \{Kristin L.\} and Yang, \{Chun Xia\} and Yolken, \{Robert H.\} and Severance, \{Emily G.\} and Lorraine Jones-Brando and Pletnikov, \{Mikhail V.\}",

year = "2014",

month = oct,

doi = "10.1016/j.exppara.2014.08.001",

language = "English",

volume = "145",

pages = "110--117",

journal = "Experimental Parasitology",

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publisher = "Academic Press Inc.",

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}

Kannan, G, Prandovszky, E, Steinfeldt, CB, Gressitt, KL, Yang, CX, Yolken, RH, Severance, EG, Jones-Brando, L & Pletnikov, MV 2014, 'One minute ultraviolet exposure inhibits Toxoplasma gondii tachyzoite replication and cyst conversion without diminishing host humoral-mediated immune response', Experimental Parasitology, vol. 145, no. 1, pp. 110-117. https://doi.org/10.1016/j.exppara.2014.08.001

TY - JOUR

T1 - One minute ultraviolet exposure inhibits Toxoplasma gondii tachyzoite replication and cyst conversion without diminishing host humoral-mediated immune response

AU - Kannan, Geetha

AU - Prandovszky, Emese

AU - Steinfeldt, Curtis B.

AU - Gressitt, Kristin L.

AU - Yang, Chun Xia

AU - Yolken, Robert H.

AU - Severance, Emily G.

AU - Jones-Brando, Lorraine

AU - Pletnikov, Mikhail V.

PY - 2014/10

Y1 - 2014/10

N2 - We developed a protocol to inactivate Toxoplasma gondii (T. gondii) tachyzoites employing 1. min of ultraviolet (UV) exposure. We show that this treatment completely inhibited parasite replication and cyst formation in vitro and in vivo but did not affect the induction of a robust IgG response in mice. We propose that our protocol can be used to study the contribution of the humoral immune response to rodent behavioral alterations following T. gondii infection.

AB - We developed a protocol to inactivate Toxoplasma gondii (T. gondii) tachyzoites employing 1. min of ultraviolet (UV) exposure. We show that this treatment completely inhibited parasite replication and cyst formation in vitro and in vivo but did not affect the induction of a robust IgG response in mice. We propose that our protocol can be used to study the contribution of the humoral immune response to rodent behavioral alterations following T. gondii infection.

KW - Animal models

KW - Behavior

KW - Cysts

KW - Immune response

KW - Inactivation

KW - Toxoplasma

UR - https://www.scopus.com/pages/publications/84908329006

U2 - 10.1016/j.exppara.2014.08.001

DO - 10.1016/j.exppara.2014.08.001

M3 - Article

C2 - 25131777

AN - SCOPUS:84908329006

SN - 0014-4894

VL - 145

SP - 110

EP - 117

JO - Experimental Parasitology

JF - Experimental Parasitology

IS - 1

ER -

One minute ultraviolet exposure inhibits Toxoplasma gondii tachyzoite replication and cyst conversion without diminishing host humoral-mediated immune response

Abstract

Keywords

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