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On-chip procedures for magnetic particle-based assay in droplets

  • SUNY Buffalo

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We propose on-chip procedures for magnetic particle-based assay in droplets in which excess reagents are removed more than a factor of 1/16 by extracting the magnetic particles into a washing buffer droplet, applying a magnetic field from a permanent magnet. For proof of concept, it is demonstrated to perform immunoassay between streptavidin conjugated 011 the surface of the magnetic particles and biotin labeled with fluorescence. We expect that by varying the surface functionality of the magnetic particles, the proposed method could be used to perform many useful and analytical immunoassay researches in the continuous flow droplet-based microfluidic platform.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages347-349
Number of pages3
ISBN (Print)9780979806452
StatePublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/TerritoryJapan
CityOkinawa
Period10/28/1211/1/12

Keywords

  • Continuous flow
  • Droplet
  • Magnetic particle-based assay
  • Washing

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