Nuclear localization of functional FGF receptor 1 in human astrocytes suggests a novel mechanism for growth factor action

M. K. Stachowiak; P. A. Maher; A. Joy; E. Mordechai; E. K. Stachowiak

doi:10.1016/0169-328X(96)00010-1

Nuclear localization of functional FGF receptor 1 in human astrocytes suggests a novel mechanism for growth factor action

M. K. Stachowiak
, P. A. Maher
, A. Joy
, E. Mordechai
, E. K. Stachowiak

Department of Pathology and Anatomical Sciences

Scripps Research Institute
St. Joseph's Hospital and Medical Center, Phoenix

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

Fractionation of human astrocytes revealed the presence of 103, 118, and 145 kDa forms of FGF receptor 1 (FGFR1) in isolated nuclei. Only trace amounts of FGFR1 proteins were detected in the cell membrane or cytoplasmic fractions. Nuclear FGFR1 is found in the nucleoplasm and nuclear matrix but not in chromatin. Immuno-confocal microscopy further demonstrates the intranuclear presence of FGFR1 and its colocalization with FGF-2. Nuclear FGFR1 binds to FGF-2 and has tyrosine kinase activity. Translocation of functional growth factor receptors into the cell nucleus offers a novel mechanism for growth factor action.

Original language	English
Pages (from-to)	161-165
Number of pages	5
Journal	Molecular Brain Research
Volume	38
Issue number	1
DOIs	https://doi.org/10.1016/0169-328X(96)00010-1
State	Published - May 1996

Keywords

Astrocyte
Fibroblast growth factor receptors
Human
Nucleus

Access to Document

10.1016/0169-328X(96)00010-1

Cite this

@article{346cb359a64449c4abe3824440601985,

title = "Nuclear localization of functional FGF receptor 1 in human astrocytes suggests a novel mechanism for growth factor action",

abstract = "Fractionation of human astrocytes revealed the presence of 103, 118, and 145 kDa forms of FGF receptor 1 (FGFR1) in isolated nuclei. Only trace amounts of FGFR1 proteins were detected in the cell membrane or cytoplasmic fractions. Nuclear FGFR1 is found in the nucleoplasm and nuclear matrix but not in chromatin. Immuno-confocal microscopy further demonstrates the intranuclear presence of FGFR1 and its colocalization with FGF-2. Nuclear FGFR1 binds to FGF-2 and has tyrosine kinase activity. Translocation of functional growth factor receptors into the cell nucleus offers a novel mechanism for growth factor action.",

keywords = "Astrocyte, Fibroblast growth factor receptors, Human, Nucleus",

author = "Stachowiak, \{M. K.\} and Maher, \{P. A.\} and A. Joy and E. Mordechai and Stachowiak, \{E. K.\}",

year = "1996",

month = may,

doi = "10.1016/0169-328X(96)00010-1",

language = "English",

volume = "38",

pages = "161--165",

journal = "Molecular Brain Research",

issn = "0169-328X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Nuclear localization of functional FGF receptor 1 in human astrocytes suggests a novel mechanism for growth factor action

AU - Stachowiak, M. K.

AU - Maher, P. A.

AU - Joy, A.

AU - Mordechai, E.

AU - Stachowiak, E. K.

PY - 1996/5

Y1 - 1996/5

N2 - Fractionation of human astrocytes revealed the presence of 103, 118, and 145 kDa forms of FGF receptor 1 (FGFR1) in isolated nuclei. Only trace amounts of FGFR1 proteins were detected in the cell membrane or cytoplasmic fractions. Nuclear FGFR1 is found in the nucleoplasm and nuclear matrix but not in chromatin. Immuno-confocal microscopy further demonstrates the intranuclear presence of FGFR1 and its colocalization with FGF-2. Nuclear FGFR1 binds to FGF-2 and has tyrosine kinase activity. Translocation of functional growth factor receptors into the cell nucleus offers a novel mechanism for growth factor action.

AB - Fractionation of human astrocytes revealed the presence of 103, 118, and 145 kDa forms of FGF receptor 1 (FGFR1) in isolated nuclei. Only trace amounts of FGFR1 proteins were detected in the cell membrane or cytoplasmic fractions. Nuclear FGFR1 is found in the nucleoplasm and nuclear matrix but not in chromatin. Immuno-confocal microscopy further demonstrates the intranuclear presence of FGFR1 and its colocalization with FGF-2. Nuclear FGFR1 binds to FGF-2 and has tyrosine kinase activity. Translocation of functional growth factor receptors into the cell nucleus offers a novel mechanism for growth factor action.

KW - Astrocyte

KW - Fibroblast growth factor receptors

KW - Human

KW - Nucleus

UR - https://www.scopus.com/pages/publications/0029997013

U2 - 10.1016/0169-328X(96)00010-1

DO - 10.1016/0169-328X(96)00010-1

M3 - Article

C2 - 8737680

AN - SCOPUS:0029997013

SN - 0169-328X

VL - 38

SP - 161

EP - 165

JO - Molecular Brain Research

JF - Molecular Brain Research

IS - 1

ER -

Nuclear localization of functional FGF receptor 1 in human astrocytes suggests a novel mechanism for growth factor action

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this