Nuclear actin and myosin I are required for RNA polymerase I transcription

Vlada V. Philimonenko; Jian Zhao; Sebastian Iben; Hana Dingová; Katarína Kyselá; Michal Kahle; Hanswalter Zentgraf; Wilma A. Hofmann; Primal de Lanerolle; Pavel Hozák; Ingrid Grummt

doi:10.1038/ncb1190

Nuclear actin and myosin I are required for RNA polymerase I transcription

Vlada V. Philimonenko
, Jian Zhao
, Sebastian Iben
, Hana Dingová
, Katarína Kyselá
, Michal Kahle
, Hanswalter Zentgraf
, Wilma A. Hofmann
, Primal de Lanerolle
, Pavel Hozák
, Ingrid Grummt

Physiology and Biophysics

Czech Academy of Sciences
German Cancer Research Center
University of Illinois at Chicago

Research output: Contribution to journal › Article › peer-review

360 Scopus citations

Abstract

The presence of actin and nuclear myosin I (NMI) in the nucleus suggests a role for these motor proteins in nuclear functions. We have investigated the role of actin and nuclear myosin I (NMI) in the transcription of ribosomal RNA genes (rDNA). Both proteins are associated with rDNA and are required for RNA polymerase I (Pol I) transcription. Microinjection of antibodies against actin or NMI, as well as short interfering RNA-mediated depletion of NMI, decreased Pol I transcription in vivo, whereas overexpression of NMI augmented pre-rRNA synthesis. In vitro, recombinant NMI activated Pol I transcription, and antibodies to NMI or actin inhibited Pol I transcription both on naked DNA and pre-assembled chromatin templates. Whereas actin associated with Pol I, NMI bound to Pol I through the transcription-initiation factor TIF-IA. The association with Pol I requires phosphorylation of TIF-IA at Ser 649 by RSK kinase, indicating a role for NMI in the growth-dependent regulation of rRNA synthesis.

Original language	English
Pages (from-to)	1165-1172
Number of pages	8
Journal	Nature Cell Biology
Volume	6
Issue number	12
DOIs	https://doi.org/10.1038/ncb1190
State	Published - Dec 2004

Access to Document

10.1038/ncb1190

Cite this

@article{aeb27bc3d19747319a952cddcc528775,

title = "Nuclear actin and myosin I are required for RNA polymerase I transcription",

abstract = "The presence of actin and nuclear myosin I (NMI) in the nucleus suggests a role for these motor proteins in nuclear functions. We have investigated the role of actin and nuclear myosin I (NMI) in the transcription of ribosomal RNA genes (rDNA). Both proteins are associated with rDNA and are required for RNA polymerase I (Pol I) transcription. Microinjection of antibodies against actin or NMI, as well as short interfering RNA-mediated depletion of NMI, decreased Pol I transcription in vivo, whereas overexpression of NMI augmented pre-rRNA synthesis. In vitro, recombinant NMI activated Pol I transcription, and antibodies to NMI or actin inhibited Pol I transcription both on naked DNA and pre-assembled chromatin templates. Whereas actin associated with Pol I, NMI bound to Pol I through the transcription-initiation factor TIF-IA. The association with Pol I requires phosphorylation of TIF-IA at Ser 649 by RSK kinase, indicating a role for NMI in the growth-dependent regulation of rRNA synthesis.",

author = "Philimonenko, \{Vlada V.\} and Jian Zhao and Sebastian Iben and Hana Dingov{\'a} and Katar{\'i}na Kysel{\'a} and Michal Kahle and Hanswalter Zentgraf and Hofmann, \{Wilma A.\} and \{de Lanerolle\}, Primal and Pavel Hoz{\'a}k and Ingrid Grummt",

year = "2004",

month = dec,

doi = "10.1038/ncb1190",

language = "English",

volume = "6",

pages = "1165--1172",

journal = "Nature Cell Biology",

issn = "1465-7392",

publisher = "Nature Research",

number = "12",

}

TY - JOUR

T1 - Nuclear actin and myosin I are required for RNA polymerase I transcription

AU - Philimonenko, Vlada V.

AU - Zhao, Jian

AU - Iben, Sebastian

AU - Dingová, Hana

AU - Kyselá, Katarína

AU - Kahle, Michal

AU - Zentgraf, Hanswalter

AU - Hofmann, Wilma A.

AU - de Lanerolle, Primal

AU - Hozák, Pavel

AU - Grummt, Ingrid

PY - 2004/12

Y1 - 2004/12

N2 - The presence of actin and nuclear myosin I (NMI) in the nucleus suggests a role for these motor proteins in nuclear functions. We have investigated the role of actin and nuclear myosin I (NMI) in the transcription of ribosomal RNA genes (rDNA). Both proteins are associated with rDNA and are required for RNA polymerase I (Pol I) transcription. Microinjection of antibodies against actin or NMI, as well as short interfering RNA-mediated depletion of NMI, decreased Pol I transcription in vivo, whereas overexpression of NMI augmented pre-rRNA synthesis. In vitro, recombinant NMI activated Pol I transcription, and antibodies to NMI or actin inhibited Pol I transcription both on naked DNA and pre-assembled chromatin templates. Whereas actin associated with Pol I, NMI bound to Pol I through the transcription-initiation factor TIF-IA. The association with Pol I requires phosphorylation of TIF-IA at Ser 649 by RSK kinase, indicating a role for NMI in the growth-dependent regulation of rRNA synthesis.

AB - The presence of actin and nuclear myosin I (NMI) in the nucleus suggests a role for these motor proteins in nuclear functions. We have investigated the role of actin and nuclear myosin I (NMI) in the transcription of ribosomal RNA genes (rDNA). Both proteins are associated with rDNA and are required for RNA polymerase I (Pol I) transcription. Microinjection of antibodies against actin or NMI, as well as short interfering RNA-mediated depletion of NMI, decreased Pol I transcription in vivo, whereas overexpression of NMI augmented pre-rRNA synthesis. In vitro, recombinant NMI activated Pol I transcription, and antibodies to NMI or actin inhibited Pol I transcription both on naked DNA and pre-assembled chromatin templates. Whereas actin associated with Pol I, NMI bound to Pol I through the transcription-initiation factor TIF-IA. The association with Pol I requires phosphorylation of TIF-IA at Ser 649 by RSK kinase, indicating a role for NMI in the growth-dependent regulation of rRNA synthesis.

UR - https://www.scopus.com/pages/publications/10344264971

U2 - 10.1038/ncb1190

DO - 10.1038/ncb1190

M3 - Article

C2 - 15558034

AN - SCOPUS:10344264971

SN - 1465-7392

VL - 6

SP - 1165

EP - 1172

JO - Nature Cell Biology

JF - Nature Cell Biology

IS - 12

ER -

Nuclear actin and myosin I are required for RNA polymerase I transcription

Abstract

Access to Document

Other files and links

Fingerprint

Cite this