New species of tyrosine tRNA in nonsense suppressor strains of yeast

Jeremy Bruenn; K. Bruce Jacobson

doi:10.1016/0005-2787(72)90330-9

New species of tyrosine tRNA in nonsense suppressor strains of yeast

Jeremy Bruenn
, K. Bruce Jacobson

Biological Sciences

University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A number of mutations in Saccharomyces cerevisiae are subject to suppression in so-called supersuppressor strains. The tyrosyl-tRNAs from two Class I, Set I ochre-specific suppressor strains, in which UAA is read as tyrosine, have been examined by reversed-phase column chromatography. In each ochre-specific strain there is an extra early-chromatographing tyrosyl-tRNA species present that is not present in wild type. Strains carrying an amber suppressor gene that is allelic to one of these ochre-specific suppressors possess a new late-chromatographing tyrosyl-tRNA not present in the wild type. In addition, strains with either the amber suppressor or its allelic ochre-specific suppressor lack a late-chromatographing portion of the wild-type tyrosyl-tRNA. This may indicate that the new tyrosyl-tRNAs are derived from but one of several presumed components of the main peak of this tRNA.

Original language	English
Pages (from-to)	68-76
Number of pages	9
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	287
Issue number	1
DOIs	https://doi.org/10.1016/0005-2787(72)90330-9
State	Published - Nov 16 1972

Access to Document

10.1016/0005-2787(72)90330-9

Cite this

@article{e4643600f6d24a00be7b64683208ef6f,

title = "New species of tyrosine tRNA in nonsense suppressor strains of yeast",

abstract = "A number of mutations in Saccharomyces cerevisiae are subject to suppression in so-called supersuppressor strains. The tyrosyl-tRNAs from two Class I, Set I ochre-specific suppressor strains, in which UAA is read as tyrosine, have been examined by reversed-phase column chromatography. In each ochre-specific strain there is an extra early-chromatographing tyrosyl-tRNA species present that is not present in wild type. Strains carrying an amber suppressor gene that is allelic to one of these ochre-specific suppressors possess a new late-chromatographing tyrosyl-tRNA not present in the wild type. In addition, strains with either the amber suppressor or its allelic ochre-specific suppressor lack a late-chromatographing portion of the wild-type tyrosyl-tRNA. This may indicate that the new tyrosyl-tRNAs are derived from but one of several presumed components of the main peak of this tRNA.",

author = "Jeremy Bruenn and Jacobson, \{K. Bruce\}",

year = "1972",

month = nov,

day = "16",

doi = "10.1016/0005-2787(72)90330-9",

language = "English",

volume = "287",

pages = "68--76",

journal = "BBA Section Nucleic Acids And Protein Synthesis",

issn = "0005-2787",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - New species of tyrosine tRNA in nonsense suppressor strains of yeast

AU - Bruenn, Jeremy

AU - Jacobson, K. Bruce

PY - 1972/11/16

Y1 - 1972/11/16

N2 - A number of mutations in Saccharomyces cerevisiae are subject to suppression in so-called supersuppressor strains. The tyrosyl-tRNAs from two Class I, Set I ochre-specific suppressor strains, in which UAA is read as tyrosine, have been examined by reversed-phase column chromatography. In each ochre-specific strain there is an extra early-chromatographing tyrosyl-tRNA species present that is not present in wild type. Strains carrying an amber suppressor gene that is allelic to one of these ochre-specific suppressors possess a new late-chromatographing tyrosyl-tRNA not present in the wild type. In addition, strains with either the amber suppressor or its allelic ochre-specific suppressor lack a late-chromatographing portion of the wild-type tyrosyl-tRNA. This may indicate that the new tyrosyl-tRNAs are derived from but one of several presumed components of the main peak of this tRNA.

AB - A number of mutations in Saccharomyces cerevisiae are subject to suppression in so-called supersuppressor strains. The tyrosyl-tRNAs from two Class I, Set I ochre-specific suppressor strains, in which UAA is read as tyrosine, have been examined by reversed-phase column chromatography. In each ochre-specific strain there is an extra early-chromatographing tyrosyl-tRNA species present that is not present in wild type. Strains carrying an amber suppressor gene that is allelic to one of these ochre-specific suppressors possess a new late-chromatographing tyrosyl-tRNA not present in the wild type. In addition, strains with either the amber suppressor or its allelic ochre-specific suppressor lack a late-chromatographing portion of the wild-type tyrosyl-tRNA. This may indicate that the new tyrosyl-tRNAs are derived from but one of several presumed components of the main peak of this tRNA.

UR - https://www.scopus.com/pages/publications/0015512983

U2 - 10.1016/0005-2787(72)90330-9

DO - 10.1016/0005-2787(72)90330-9

M3 - Article

C2 - 4569159

AN - SCOPUS:0015512983

SN - 0005-2787

VL - 287

SP - 68

EP - 76

JO - BBA Section Nucleic Acids And Protein Synthesis

JF - BBA Section Nucleic Acids And Protein Synthesis

IS - 1

ER -

New species of tyrosine tRNA in nonsense suppressor strains of yeast

Abstract

Access to Document

Other files and links

Fingerprint

Cite this