Neurotrophic effects of amnion explants co-cultured with rat mesencephalon

Gail M. Seigel; Fred Junn; Don M. Gash

doi:10.1016/0165-3806(94)90221-6

Neurotrophic effects of amnion explants co-cultured with rat mesencephalon

Gail M. Seigel
, Fred Junn
, Don M. Gash

Communicative Disorders and Sciences

University of Rochester

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We investigated amnion-derived neurotrophic effects on embryonic day 14 rat mesencephalic (E14M) explants in co-culture. E14M explants showed extensive neurite outgrowth directed toward amnion tissue. Tyrosine hydroxylase immunocytochemistry, western immunoblot and [³H]dopamine uptake studies revealed significant neurotrophic effects on E14M dopaminergic neurons over 14 days in culture. Thus, amnion tissue appears to have potent neurotrophic effects for embryonic mesencephalic dopaminergic neurons in vitro.

Original language	English
Pages (from-to)	137-139
Number of pages	3
Journal	Developmental Brain Research
Volume	77
Issue number	1
DOIs	https://doi.org/10.1016/0165-3806(94)90221-6
State	Published - Jan 14 1994

Keywords

Dopamine
Neurite outgrowth
Tyrosine hydroxylase

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10.1016/0165-3806(94)90221-6

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title = "Neurotrophic effects of amnion explants co-cultured with rat mesencephalon",

abstract = "We investigated amnion-derived neurotrophic effects on embryonic day 14 rat mesencephalic (E14M) explants in co-culture. E14M explants showed extensive neurite outgrowth directed toward amnion tissue. Tyrosine hydroxylase immunocytochemistry, western immunoblot and [3H]dopamine uptake studies revealed significant neurotrophic effects on E14M dopaminergic neurons over 14 days in culture. Thus, amnion tissue appears to have potent neurotrophic effects for embryonic mesencephalic dopaminergic neurons in vitro.",

keywords = "Dopamine, Neurite outgrowth, Tyrosine hydroxylase",

author = "Seigel, \{Gail M.\} and Fred Junn and Gash, \{Don M.\}",

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AU - Seigel, Gail M.

AU - Junn, Fred

AU - Gash, Don M.

PY - 1994/1/14

Y1 - 1994/1/14

N2 - We investigated amnion-derived neurotrophic effects on embryonic day 14 rat mesencephalic (E14M) explants in co-culture. E14M explants showed extensive neurite outgrowth directed toward amnion tissue. Tyrosine hydroxylase immunocytochemistry, western immunoblot and [3H]dopamine uptake studies revealed significant neurotrophic effects on E14M dopaminergic neurons over 14 days in culture. Thus, amnion tissue appears to have potent neurotrophic effects for embryonic mesencephalic dopaminergic neurons in vitro.

AB - We investigated amnion-derived neurotrophic effects on embryonic day 14 rat mesencephalic (E14M) explants in co-culture. E14M explants showed extensive neurite outgrowth directed toward amnion tissue. Tyrosine hydroxylase immunocytochemistry, western immunoblot and [3H]dopamine uptake studies revealed significant neurotrophic effects on E14M dopaminergic neurons over 14 days in culture. Thus, amnion tissue appears to have potent neurotrophic effects for embryonic mesencephalic dopaminergic neurons in vitro.

KW - Dopamine

KW - Neurite outgrowth

KW - Tyrosine hydroxylase

UR - https://www.scopus.com/pages/publications/0028012307

U2 - 10.1016/0165-3806(94)90221-6

DO - 10.1016/0165-3806(94)90221-6

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AN - SCOPUS:0028012307

SN - 0165-3806

VL - 77

SP - 137

EP - 139

JO - Developmental Brain Research

JF - Developmental Brain Research

IS - 1

ER -

Neurotrophic effects of amnion explants co-cultured with rat mesencephalon

Abstract

Keywords

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