Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone

Liang Chen; Kalyan D. Chavda; José R. Mediavilla; Yanan Zhao; Henry S. Fraimow; Stephen G. Jenkins; Michael H. Levi; Tao Hong; Albert D. Rojtman; Christine C. Ginocchio; Robert A. Bonomo; Barry N. Kreiswirth

doi:10.1128/AAC.00316-12

Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone

Liang Chen
, Kalyan D. Chavda
, José R. Mediavilla
, Yanan Zhao
, Henry S. Fraimow
, Stephen G. Jenkins
, Michael H. Levi
, Tao Hong
, Albert D. Rojtman
, Christine C. Ginocchio
, Robert A. Bonomo
, Barry N. Kreiswirth

Pharmacy

Public Health Research Institute, New York
Cooper University Health Care
Cornell University
Albert Einstein College of Medicine
Hackensack University Medical Center
Jersey Shore University Medical Center
Northwell Health System
Louis Stokes Cleveland VA Medical Center

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and bla_KPC carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and bla_KPC in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.

Original language	English
Pages (from-to)	3444-3447
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	56
Issue number	6
DOIs	https://doi.org/10.1128/AAC.00316-12
State	Published - Jun 2012

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Chen, L., Chavda, K. D., Mediavilla, J. R., Zhao, Y., Fraimow, H. S., Jenkins, S. G., Levi, M. H., Hong, T., Rojtman, A. D., Ginocchio, C. C., Bonomo, R. A., & Kreiswirth, B. N. (2012). Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone. Antimicrobial Agents and Chemotherapy, 56(6), 3444-3447. https://doi.org/10.1128/AAC.00316-12

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title = "Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone",

abstract = "We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and blaKPC carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100\%) and specificity (100\%) for identification of ST258 clone and blaKPC in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.",

author = "Liang Chen and Chavda, \{Kalyan D.\} and Mediavilla, \{Jos{\'e} R.\} and Yanan Zhao and Fraimow, \{Henry S.\} and Jenkins, \{Stephen G.\} and Levi, \{Michael H.\} and Tao Hong and Rojtman, \{Albert D.\} and Ginocchio, \{Christine C.\} and Bonomo, \{Robert A.\} and Kreiswirth, \{Barry N.\}",

year = "2012",

month = jun,

doi = "10.1128/AAC.00316-12",

language = "English",

volume = "56",

pages = "3444--3447",

journal = "Antimicrobial Agents and Chemotherapy",

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AU - Chen, Liang

AU - Chavda, Kalyan D.

AU - Mediavilla, José R.

AU - Zhao, Yanan

AU - Fraimow, Henry S.

AU - Jenkins, Stephen G.

AU - Levi, Michael H.

AU - Hong, Tao

AU - Rojtman, Albert D.

AU - Ginocchio, Christine C.

AU - Bonomo, Robert A.

AU - Kreiswirth, Barry N.

PY - 2012/6

Y1 - 2012/6

N2 - We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and blaKPC carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and blaKPC in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.

AB - We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and blaKPC carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and blaKPC in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.

UR - https://www.scopus.com/pages/publications/84861146913

U2 - 10.1128/AAC.00316-12

DO - 10.1128/AAC.00316-12

M3 - Article

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AN - SCOPUS:84861146913

SN - 0066-4804

VL - 56

SP - 3444

EP - 3447

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 6

ER -

Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone

Abstract

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