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Multimodal assessment of spatial distribution of drug-tracer uptake by brain tissue after intra-arterial injections

  • Rajinder Singh-Moon
  • , Durba Chaudhuri
  • , Mei Wang
  • , Robert Straubinger
  • , Irving J. Bigio
  • , Shailendra Joshi
  • Columbia University
  • Boston University

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

It is challenging to track the rapid changes in drug concentrations after intra-arterial (IA) administration to elucidate the pharmacokinetics of this method of drug delivery. Traditional pharmacokinetic parameters (such as protein binding) that are highly relevant to intravenous (IV) administration do not seem to apply to IA injections. Regional drug delivery is affected by the biomechanics of drug injection, resting blood flow, and local tissue extraction. In-vivo and ex-vivo, optical methods for spatial mapping of drug deposition can assist in visualizing drug distributions and aid in the screening of potential drugs and carrier candidates. We present a multimodal approach for the assessment of drug distribution in postmortem tissue specimens using diffuse reflectance spectroscopy, multispectral imaging, and confocal microscopy and demonstrate feasibility of distinguishing route of administration advantages of liposome-dye conjugate delivery. The results of this study suggest that insight on drug dynamics gained by this aggregated approach can be used to help screen and/or optimize potential drug candidates and drug delivery protocols.

Original languageEnglish
Title of host publicationOptical Diagnostics and Sensing XIV
Subtitle of host publicationToward Point-of-Care Diagnostics
PublisherSPIE
ISBN (Print)9780819498649
DOIs
StatePublished - 2014
EventOptical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics - San Francisco, CA, United States
Duration: Feb 3 2014Feb 6 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8951
ISSN (Print)1605-7422

Conference

ConferenceOptical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics
Country/TerritoryUnited States
CitySan Francisco, CA
Period02/3/1402/6/14

Keywords

  • confocal microscopy
  • Diffuse reflectance spectroscopy
  • intra-arterial
  • liposomes
  • multispectral imaging
  • optical pharmacokinetics

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