Multi-photon micro-spectroscopy of biological specimens

Bai Ling Lin; Fu Jen Kao; Ping chin Cheng; Chi Kuang Sun; Rang Wu Chen; Yi min Wang; Jian cheng Chen; Yung Shun Wang; Tzu Ming Liu; Mao Kuo Huang

doi:10.1117/12.390530

Multi-photon micro-spectroscopy of biological specimens

Bai Ling Lin
, Fu Jen Kao
, Ping chin Cheng
, Chi Kuang Sun
, Rang Wu Chen
, Yi min Wang
, Jian cheng Chen
, Yung Shun Wang
, Tzu Ming Liu
, Mao Kuo Huang

Department of Electrical Engineering

Academia Sinica Taiwan HQ

Research output: Contribution to journal › Conference article › peer-review

3 Scopus citations

Abstract

The non-linear nature of multi-photon fluorescence excitation restricts the fluorescing volume to the vicinity of the focal point. As a result, the technology has the capacity for micro-spectroscopy of biological specimen at high spatial resolution. Mesophyll protoplasts of Arabidopsis thaliana and maize stem sections were used to demonstrate the feasibility of multi-photon fluorescence micro-spectroscopy at subcellular compartments. Time-lapse spectral recording provides a means for studying the response of cell organelles to high intensity illumination.

Original language	English
Pages (from-to)	100-104
Number of pages	5
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4082
DOIs	https://doi.org/10.1117/12.390530
State	Published - 2000
Event	Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications - Taipei, Taiwan Duration: Jul 26 2000 → Jul 28 2000

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title = "Multi-photon micro-spectroscopy of biological specimens",

abstract = "The non-linear nature of multi-photon fluorescence excitation restricts the fluorescing volume to the vicinity of the focal point. As a result, the technology has the capacity for micro-spectroscopy of biological specimen at high spatial resolution. Mesophyll protoplasts of Arabidopsis thaliana and maize stem sections were used to demonstrate the feasibility of multi-photon fluorescence micro-spectroscopy at subcellular compartments. Time-lapse spectral recording provides a means for studying the response of cell organelles to high intensity illumination.",

author = "Lin, \{Bai Ling\} and Kao, \{Fu Jen\} and Cheng, \{Ping chin\} and Sun, \{Chi Kuang\} and Chen, \{Rang Wu\} and Wang, \{Yi min\} and Chen, \{Jian cheng\} and Wang, \{Yung Shun\} and Liu, \{Tzu Ming\} and Huang, \{Mao Kuo\}",

year = "2000",

doi = "10.1117/12.390530",

language = "English",

volume = "4082",

pages = "100--104",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications ; Conference date: 26-07-2000 Through 28-07-2000",

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TY - JOUR

T1 - Multi-photon micro-spectroscopy of biological specimens

AU - Lin, Bai Ling

AU - Kao, Fu Jen

AU - Cheng, Ping chin

AU - Sun, Chi Kuang

AU - Chen, Rang Wu

AU - Wang, Yi min

AU - Chen, Jian cheng

AU - Wang, Yung Shun

AU - Liu, Tzu Ming

AU - Huang, Mao Kuo

PY - 2000

Y1 - 2000

N2 - The non-linear nature of multi-photon fluorescence excitation restricts the fluorescing volume to the vicinity of the focal point. As a result, the technology has the capacity for micro-spectroscopy of biological specimen at high spatial resolution. Mesophyll protoplasts of Arabidopsis thaliana and maize stem sections were used to demonstrate the feasibility of multi-photon fluorescence micro-spectroscopy at subcellular compartments. Time-lapse spectral recording provides a means for studying the response of cell organelles to high intensity illumination.

AB - The non-linear nature of multi-photon fluorescence excitation restricts the fluorescing volume to the vicinity of the focal point. As a result, the technology has the capacity for micro-spectroscopy of biological specimen at high spatial resolution. Mesophyll protoplasts of Arabidopsis thaliana and maize stem sections were used to demonstrate the feasibility of multi-photon fluorescence micro-spectroscopy at subcellular compartments. Time-lapse spectral recording provides a means for studying the response of cell organelles to high intensity illumination.

UR - https://www.scopus.com/pages/publications/0033682674

U2 - 10.1117/12.390530

DO - 10.1117/12.390530

M3 - Conference article

AN - SCOPUS:0033682674

SN - 0277-786X

VL - 4082

SP - 100

EP - 104

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications

Y2 - 26 July 2000 through 28 July 2000

ER -

Multi-photon micro-spectroscopy of biological specimens

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