Multi-photon fluorescence spectroscopy of fluorescent bio-probes and bio-molecules

Ping chin Cheng; Bai Ling Lin; Fu Jen Kao; Chi Kuang Sun; Yung Sheng Wang; Tzu Ming Liu; Yi min Wang; Jian cheng Chen; Mao Kuo Huang; I. Johnson

Multi-photon fluorescence spectroscopy of fluorescent bio-probes and bio-molecules

Ping chin Cheng
, Bai Ling Lin
, Fu Jen Kao
, Chi Kuang Sun
, Yung Sheng Wang
, Tzu Ming Liu
, Yi min Wang
, Jian cheng Chen
, Mao Kuo Huang
, I. Johnson

Department of Electrical Engineering

SUNY Buffalo

Research output: Contribution to journal › Conference article › peer-review

8 Scopus citations

Abstract

Multi-photon fluorescence spectra of a number of commonly used biological probes were measured in this study. Significant spectral variation has been detected between single and multi-photon excitation. The result is important for the proper selection of spectral setting/dichroic beam splitter in the set-up of a multi-photon fluorescence microscope. The information can also be useful in the detection of multi-photon fluorescence in bio-chip technology. In addition, we have investigated a few highly fluorescent bio-molecules commonly found in plant cells.

Original language	English
Pages (from-to)	87-91
Number of pages	5
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4082
State	Published - 2000
Event	Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications - Taipei, Taiwan Duration: Jul 26 2000 → Jul 28 2000

Cite this

@article{44292969e6d7470dbfa5e28a3ff39e1b,

title = "Multi-photon fluorescence spectroscopy of fluorescent bio-probes and bio-molecules",

abstract = "Multi-photon fluorescence spectra of a number of commonly used biological probes were measured in this study. Significant spectral variation has been detected between single and multi-photon excitation. The result is important for the proper selection of spectral setting/dichroic beam splitter in the set-up of a multi-photon fluorescence microscope. The information can also be useful in the detection of multi-photon fluorescence in bio-chip technology. In addition, we have investigated a few highly fluorescent bio-molecules commonly found in plant cells.",

author = "Cheng, \{Ping chin\} and Lin, \{Bai Ling\} and Kao, \{Fu Jen\} and Sun, \{Chi Kuang\} and Wang, \{Yung Sheng\} and Liu, \{Tzu Ming\} and Wang, \{Yi min\} and Chen, \{Jian cheng\} and Huang, \{Mao Kuo\} and I. Johnson",

year = "2000",

language = "English",

volume = "4082",

pages = "87--91",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications ; Conference date: 26-07-2000 Through 28-07-2000",

}

TY - JOUR

T1 - Multi-photon fluorescence spectroscopy of fluorescent bio-probes and bio-molecules

AU - Cheng, Ping chin

AU - Lin, Bai Ling

AU - Kao, Fu Jen

AU - Sun, Chi Kuang

AU - Wang, Yung Sheng

AU - Liu, Tzu Ming

AU - Wang, Yi min

AU - Chen, Jian cheng

AU - Huang, Mao Kuo

AU - Johnson, I.

PY - 2000

Y1 - 2000

N2 - Multi-photon fluorescence spectra of a number of commonly used biological probes were measured in this study. Significant spectral variation has been detected between single and multi-photon excitation. The result is important for the proper selection of spectral setting/dichroic beam splitter in the set-up of a multi-photon fluorescence microscope. The information can also be useful in the detection of multi-photon fluorescence in bio-chip technology. In addition, we have investigated a few highly fluorescent bio-molecules commonly found in plant cells.

AB - Multi-photon fluorescence spectra of a number of commonly used biological probes were measured in this study. Significant spectral variation has been detected between single and multi-photon excitation. The result is important for the proper selection of spectral setting/dichroic beam splitter in the set-up of a multi-photon fluorescence microscope. The information can also be useful in the detection of multi-photon fluorescence in bio-chip technology. In addition, we have investigated a few highly fluorescent bio-molecules commonly found in plant cells.

UR - https://www.scopus.com/pages/publications/0033697828

M3 - Conference article

AN - SCOPUS:0033697828

SN - 0277-786X

VL - 4082

SP - 87

EP - 91

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications

Y2 - 26 July 2000 through 28 July 2000

ER -

Multi-photon fluorescence spectroscopy of fluorescent bio-probes and bio-molecules

Abstract

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