Abstract
The applications of two-photon fluorescence microscopy (TPFM) in medical imaging are discussed. Strong fluorescence emission is restricted in the TPFM with the advantage of minimum out-of-focus absorption, photobleaching and damage. This microscopic technique requires in-focus non-linear absorption to stimulate fluorescence emission. The use of long illumination wavelength reduces the background auto-fluorescence and photo-damage. The light attenuation in biological specimen of human skin and plant tissues is also analyzed.
| Original language | English |
|---|---|
| Pages | 222 |
| Number of pages | 1 |
| State | Published - 2001 |
| Event | Conference on Lasers and Electro-Optics (CLEO) - Baltimore, MD, United States Duration: May 6 2001 → May 11 2001 |
Conference
| Conference | Conference on Lasers and Electro-Optics (CLEO) |
|---|---|
| Country/Territory | United States |
| City | Baltimore, MD |
| Period | 05/6/01 → 05/11/01 |
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