Abstract
We are studying peptide immunogenicity as a function of the similarity level to the host's proteome. By using as a model the breast/prostate cancer-associated HER-2/neu antigen, we analyzed the monoclonal and polyclonal humoral immune responses against HER-2/neu peptide motifs not shared with the host proteome. We show here that (i) a mouse monoclonal antibody (MAb) raised against the extra-cellular domain (EC) of human HER-2/neu oncoprotein recognized a linear peptide motif endowed with low similarity level to the mouse proteome; (ii) likewise, human sera from breast/prostate cancer patients preferentially recognized peptide fragments from the EC of the HER-2/neu oncoprotein having sequences that are not present in the human proteome. Together with previous results obtained in other disease models (cervical cancer-associated HPV16 E7 oncoprotein and Pemphigus vulgaris auto-antigen desmoglein-3), the present data suggest that a low level of sequence similarity to the host's proteome might be an important factor in shaping the pool of B cell epitopes.
| Original language | English |
|---|---|
| Pages (from-to) | 741-747 |
| Number of pages | 7 |
| Journal | International Journal of Cancer |
| Volume | 98 |
| Issue number | 5 |
| DOIs | |
| State | Published - Apr 10 2002 |
Keywords
- Breast/prostate cancer
- Epitope prediction
- HER-2/neu
- Molecular mimicry
Fingerprint
Dive into the research topics of 'Monoclonal and polyclonal humoral immune response to EC HER-2/neu peptides with low similarity to the host's proteome'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver