Molekuliarno-tsitogeneticheskiǐ podkhod k kartirovaniiu metilirovannykh polimorfnykh restriktsionnykh fragmentov genov rRNK

I. V. Gar'kavtsev; T. G. Tsvetkova; N. A. Egolina; E. V. Mkhitarova; A. V. Gudkov

Molekuliarno-tsitogeneticheskiǐ podkhod k kartirovaniiu metilirovannykh polimorfnykh restriktsionnykh fragmentov genov rRNK

Translated title of the contribution: Molecular and cytogenetic approach to the mapping of methylated polymorphic restriction fragments of human rRNA genes

I. V. Gar'kavtsev
, T. G. Tsvetkova
, N. A. Egolina
, E. V. Mkhitarova
, A. V. Gudkov

Roswell Park - Cell Stress and Biophysical Oncology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Combined restriction with Bam H I and Sal I (or Hpa II) has revealed Bam H I fragment on a non-transcribed spacer of rRNA genes in one out of four individuals under study. Using Ag-staining and hybridization in situ, chromosome 13p+ enriched by inactive rRNA gene copies was found in the given individual. Since Sal I does not restrict methylated sequences and rRNA genes are repressed by methylation, it is concluded that methylated Banm I-restricted rRNA gene fragments of non-transcribed spacer are localized in chromosome 13p+ of the individual in question.

Translated title of the contribution	Molecular and cytogenetic approach to the mapping of methylated polymorphic restriction fragments of human rRNA genes
Original language	Russian
Pages (from-to)	330-331
Number of pages	2
Journal	Byulleten Eksperimentalnoi Biologii i Meditsiny
Volume	102
Issue number	9
State	Published - Sep 1986

Cite this

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title = "Molekuliarno-tsitogeneticheskiǐ podkhod k kartirovaniiu metilirovannykh polimorfnykh restriktsionnykh fragmentov genov rRNK",

abstract = "Combined restriction with Bam H I and Sal I (or Hpa II) has revealed Bam H I fragment on a non-transcribed spacer of rRNA genes in one out of four individuals under study. Using Ag-staining and hybridization in situ, chromosome 13p+ enriched by inactive rRNA gene copies was found in the given individual. Since Sal I does not restrict methylated sequences and rRNA genes are repressed by methylation, it is concluded that methylated Banm I-restricted rRNA gene fragments of non-transcribed spacer are localized in chromosome 13p+ of the individual in question.",

author = "Gar'kavtsev, \{I. V.\} and Tsvetkova, \{T. G.\} and Egolina, \{N. A.\} and Mkhitarova, \{E. V.\} and Gudkov, \{A. V.\}",

year = "1986",

month = sep,

language = "Russian",

volume = "102",

pages = "330--331",

journal = "Byulleten Eksperimentalnoi Biologii i Meditsiny",

issn = "0365-9615",

publisher = "Rossiiskaya Akademiya Meditsinskikh Nauk",

number = "9",

}

TY - JOUR

T1 - Molekuliarno-tsitogeneticheskiǐ podkhod k kartirovaniiu metilirovannykh polimorfnykh restriktsionnykh fragmentov genov rRNK

AU - Gar'kavtsev, I. V.

AU - Tsvetkova, T. G.

AU - Egolina, N. A.

AU - Mkhitarova, E. V.

AU - Gudkov, A. V.

PY - 1986/9

Y1 - 1986/9

N2 - Combined restriction with Bam H I and Sal I (or Hpa II) has revealed Bam H I fragment on a non-transcribed spacer of rRNA genes in one out of four individuals under study. Using Ag-staining and hybridization in situ, chromosome 13p+ enriched by inactive rRNA gene copies was found in the given individual. Since Sal I does not restrict methylated sequences and rRNA genes are repressed by methylation, it is concluded that methylated Banm I-restricted rRNA gene fragments of non-transcribed spacer are localized in chromosome 13p+ of the individual in question.

AB - Combined restriction with Bam H I and Sal I (or Hpa II) has revealed Bam H I fragment on a non-transcribed spacer of rRNA genes in one out of four individuals under study. Using Ag-staining and hybridization in situ, chromosome 13p+ enriched by inactive rRNA gene copies was found in the given individual. Since Sal I does not restrict methylated sequences and rRNA genes are repressed by methylation, it is concluded that methylated Banm I-restricted rRNA gene fragments of non-transcribed spacer are localized in chromosome 13p+ of the individual in question.

UR - https://www.scopus.com/pages/publications/0022774548

M3 - Article

C2 - 2875749

AN - SCOPUS:0022774548

SN - 0365-9615

VL - 102

SP - 330

EP - 331

JO - Byulleten Eksperimentalnoi Biologii i Meditsiny

JF - Byulleten Eksperimentalnoi Biologii i Meditsiny

IS - 9

ER -

Molekuliarno-tsitogeneticheskiǐ podkhod k kartirovaniiu metilirovannykh polimorfnykh restriktsionnykh fragmentov genov rRNK

Abstract

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