Molecular cloning and physical mapping of varicella zoster virus DNA

S. E. Straus; J. Owens; W. T. Ruyechan; H. E. Takiff; T. A. Casey; G. F. Vande Woude; J. Hay

doi:10.1073/pnas.79.4.993

Molecular cloning and physical mapping of varicella zoster virus DNA

S. E. Straus
, J. Owens
, W. T. Ruyechan
, H. E. Takiff
, T. A. Casey
, G. F. Vande Woude
, J. Hay

Microbiology and Immunology

National Institutes of Health

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Varicella-zoster virus (VZV) DNA was cleaved with restriction endonuclease EcoRI, and most of the resulting fragments were successfully cloned in the phage vector λgtWES.λB. Double digestions of cloned fragments with EcoRI and BamHI and hybridizations to blot-transferred BamHI digests of VZV DNA were used to construct a physical map of the genome. The molecular termini of the DNA were identified by restriction enzyme analysis after exonuclease III digestion. The data indicate that VZV DNA exists in two isomeric forms that differ by inversion of one short terminal genome segment. Electron microscopic studies revealed that the short genome segment consists of a terminal sequence of about 3.4x10⁶ daltons that is separated from an internal inverted repeat of itself by a 5.8x10⁶-dalton unique DNA segment.

Original language	English
Pages (from-to)	993-997
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	79
Issue number	4 I
DOIs	https://doi.org/10.1073/pnas.79.4.993
State	Published - 1982

Access to Document

10.1073/pnas.79.4.993

Cite this

@article{84445b8d041c433c86a5b3796ddbe355,

title = "Molecular cloning and physical mapping of varicella zoster virus DNA",

abstract = "Varicella-zoster virus (VZV) DNA was cleaved with restriction endonuclease EcoRI, and most of the resulting fragments were successfully cloned in the phage vector λgtWES.λB. Double digestions of cloned fragments with EcoRI and BamHI and hybridizations to blot-transferred BamHI digests of VZV DNA were used to construct a physical map of the genome. The molecular termini of the DNA were identified by restriction enzyme analysis after exonuclease III digestion. The data indicate that VZV DNA exists in two isomeric forms that differ by inversion of one short terminal genome segment. Electron microscopic studies revealed that the short genome segment consists of a terminal sequence of about 3.4x106 daltons that is separated from an internal inverted repeat of itself by a 5.8x106-dalton unique DNA segment.",

author = "Straus, \{S. E.\} and J. Owens and Ruyechan, \{W. T.\} and Takiff, \{H. E.\} and Casey, \{T. A.\} and \{Vande Woude\}, \{G. F.\} and J. Hay",

year = "1982",

doi = "10.1073/pnas.79.4.993",

language = "English",

volume = "79",

pages = "993--997",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "4 I",

}

TY - JOUR

T1 - Molecular cloning and physical mapping of varicella zoster virus DNA

AU - Straus, S. E.

AU - Owens, J.

AU - Ruyechan, W. T.

AU - Takiff, H. E.

AU - Casey, T. A.

AU - Vande Woude, G. F.

AU - Hay, J.

PY - 1982

Y1 - 1982

N2 - Varicella-zoster virus (VZV) DNA was cleaved with restriction endonuclease EcoRI, and most of the resulting fragments were successfully cloned in the phage vector λgtWES.λB. Double digestions of cloned fragments with EcoRI and BamHI and hybridizations to blot-transferred BamHI digests of VZV DNA were used to construct a physical map of the genome. The molecular termini of the DNA were identified by restriction enzyme analysis after exonuclease III digestion. The data indicate that VZV DNA exists in two isomeric forms that differ by inversion of one short terminal genome segment. Electron microscopic studies revealed that the short genome segment consists of a terminal sequence of about 3.4x106 daltons that is separated from an internal inverted repeat of itself by a 5.8x106-dalton unique DNA segment.

AB - Varicella-zoster virus (VZV) DNA was cleaved with restriction endonuclease EcoRI, and most of the resulting fragments were successfully cloned in the phage vector λgtWES.λB. Double digestions of cloned fragments with EcoRI and BamHI and hybridizations to blot-transferred BamHI digests of VZV DNA were used to construct a physical map of the genome. The molecular termini of the DNA were identified by restriction enzyme analysis after exonuclease III digestion. The data indicate that VZV DNA exists in two isomeric forms that differ by inversion of one short terminal genome segment. Electron microscopic studies revealed that the short genome segment consists of a terminal sequence of about 3.4x106 daltons that is separated from an internal inverted repeat of itself by a 5.8x106-dalton unique DNA segment.

UR - https://www.scopus.com/pages/publications/0020038262

U2 - 10.1073/pnas.79.4.993

DO - 10.1073/pnas.79.4.993

M3 - Article

C2 - 6280178

AN - SCOPUS:0020038262

SN - 0027-8424

VL - 79

SP - 993

EP - 997

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 4 I

ER -

Molecular cloning and physical mapping of varicella zoster virus DNA

Abstract

Access to Document

Other files and links

Fingerprint

Cite this