Modulation of phospholipase A2 activity in zymogen granule membranes by GTP[S]; Evidence for GTP-binding protein regulation

Ronald P. Rubin; Matthew Withiam-Leitch; Suzanne G. Laychock

doi:10.1016/0006-291X(91)91942-6

Modulation of phospholipase A₂ activity in zymogen granule membranes by GTP[S]; Evidence for GTP-binding protein regulation

Ronald P. Rubin
, Matthew Withiam-Leitch
, Suzanne G. Laychock

Pharmacology and Toxicology

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

In membranes associated with purified pancreatic zymogen granules, GTP[S] elicited a concentration-dependent activation of phospholipase A₂ (PLA₂), which was converted to inhibition in the presence of added Ca²⁺. The GTP-binding protein inhibitor GDP[S] blocked both the stimulatory and inhibitory actions of GTP[S]. We conclude that in zymogen granule membranes GTP-binding proteins exert a dual regulation of PLA₂ activity.

Original language	English
Pages (from-to)	22-26
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	177
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(91)91942-6
State	Published - May 31 1991

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title = "Modulation of phospholipase A2 activity in zymogen granule membranes by GTP[S]; Evidence for GTP-binding protein regulation",

abstract = "In membranes associated with purified pancreatic zymogen granules, GTP[S] elicited a concentration-dependent activation of phospholipase A2 (PLA2), which was converted to inhibition in the presence of added Ca2+. The GTP-binding protein inhibitor GDP[S] blocked both the stimulatory and inhibitory actions of GTP[S]. We conclude that in zymogen granule membranes GTP-binding proteins exert a dual regulation of PLA2 activity.",

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AU - Withiam-Leitch, Matthew

AU - Laychock, Suzanne G.

PY - 1991/5/31

Y1 - 1991/5/31

N2 - In membranes associated with purified pancreatic zymogen granules, GTP[S] elicited a concentration-dependent activation of phospholipase A2 (PLA2), which was converted to inhibition in the presence of added Ca2+. The GTP-binding protein inhibitor GDP[S] blocked both the stimulatory and inhibitory actions of GTP[S]. We conclude that in zymogen granule membranes GTP-binding proteins exert a dual regulation of PLA2 activity.

AB - In membranes associated with purified pancreatic zymogen granules, GTP[S] elicited a concentration-dependent activation of phospholipase A2 (PLA2), which was converted to inhibition in the presence of added Ca2+. The GTP-binding protein inhibitor GDP[S] blocked both the stimulatory and inhibitory actions of GTP[S]. We conclude that in zymogen granule membranes GTP-binding proteins exert a dual regulation of PLA2 activity.

UR - https://www.scopus.com/pages/publications/0025923344

U2 - 10.1016/0006-291X(91)91942-6

DO - 10.1016/0006-291X(91)91942-6

M3 - Article

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AN - SCOPUS:0025923344

SN - 0006-291X

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JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Modulation of phospholipase A₂ activity in zymogen granule membranes by GTP[S]; Evidence for GTP-binding protein regulation

Abstract

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