Abstract
The use of first-derivative synchronous excitation fluorescence is described for the minimisation of bilirubin interference in the determination of fluorescein. The limits of detection and the standard errors of estimates are better using the synchronous first-derivative approach than with conventional subtraction of the bilirubin blank. Results are shown for the quantitation of fluorescein in the nanomole concentration range.
| Original language | English |
|---|---|
| Pages (from-to) | 205-207 |
| Number of pages | 3 |
| Journal | Analyst |
| Volume | 111 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1986 |
Keywords
- Bilirubin interference
- First-derivative synchronous excitation fluorescence
- Fluorescein determination
- Interferent elimination
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