Microsatellite-enriched genomic libraries as a source of polymorphic loci for Schistosoma mansoni: Primer note

N. B. Rodrigues; M. R. Silva; M. M. Pucci; D. J. Minchella; R. Sorensen; P. T. Loverde; A. J. Romanha; G. Oliveira

doi:10.1111/j.1471-8286.2006.01575.x

Microsatellite-enriched genomic libraries as a source of polymorphic loci for Schistosoma mansoni: Primer note

N. B. Rodrigues
, M. R. Silva
, M. M. Pucci
, D. J. Minchella
, R. Sorensen
, P. T. Loverde
, A. J. Romanha
, G. Oliveira

Microbiology and Immunology

Fundação Oswaldo Cruz
Purdue University
Minnesota State University Mankato
Programa de Pós-Graduação e Pesquisa

Research output: Contribution to journal › Review article › peer-review

19 Scopus citations

Abstract

Microsatellite markers for Schistosoma mansoni were developed using four genomic microsatellite-enriched libraries. Microsatellites were observed in 65.4% of all sequences. Primer pairs were designed and tested for 23 loci. Eighteen loci produced amplification products, out of which 11 were polymorphic and were further characterized on 100 individuals of S. mansoni. Two to 19 alleles per locus were detected. The average values of expected and observed heterozygosities among the 11 loci were 0.79 and 0.59, respectively.

Original language	English
Pages (from-to)	263-265
Number of pages	3
Journal	Molecular Ecology Notes
Volume	7
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-8286.2006.01575.x
State	Published - Mar 2007

Keywords

Computational biology
Genome
Genomic library
Microsatellites
Population genetics
Schistosoma mansoni

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10.1111/j.1471-8286.2006.01575.x

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title = "Microsatellite-enriched genomic libraries as a source of polymorphic loci for Schistosoma mansoni: Primer note",

abstract = "Microsatellite markers for Schistosoma mansoni were developed using four genomic microsatellite-enriched libraries. Microsatellites were observed in 65.4\% of all sequences. Primer pairs were designed and tested for 23 loci. Eighteen loci produced amplification products, out of which 11 were polymorphic and were further characterized on 100 individuals of S. mansoni. Two to 19 alleles per locus were detected. The average values of expected and observed heterozygosities among the 11 loci were 0.79 and 0.59, respectively.",

keywords = "Computational biology, Genome, Genomic library, Microsatellites, Population genetics, Schistosoma mansoni",

author = "Rodrigues, \{N. B.\} and Silva, \{M. R.\} and Pucci, \{M. M.\} and Minchella, \{D. J.\} and R. Sorensen and Loverde, \{P. T.\} and Romanha, \{A. J.\} and G. Oliveira",

year = "2007",

month = mar,

doi = "10.1111/j.1471-8286.2006.01575.x",

language = "English",

volume = "7",

pages = "263--265",

journal = "Molecular Ecology Notes",

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TY - JOUR

T1 - Microsatellite-enriched genomic libraries as a source of polymorphic loci for Schistosoma mansoni

T2 - Primer note

AU - Rodrigues, N. B.

AU - Silva, M. R.

AU - Pucci, M. M.

AU - Minchella, D. J.

AU - Sorensen, R.

AU - Loverde, P. T.

AU - Romanha, A. J.

AU - Oliveira, G.

PY - 2007/3

Y1 - 2007/3

N2 - Microsatellite markers for Schistosoma mansoni were developed using four genomic microsatellite-enriched libraries. Microsatellites were observed in 65.4% of all sequences. Primer pairs were designed and tested for 23 loci. Eighteen loci produced amplification products, out of which 11 were polymorphic and were further characterized on 100 individuals of S. mansoni. Two to 19 alleles per locus were detected. The average values of expected and observed heterozygosities among the 11 loci were 0.79 and 0.59, respectively.

AB - Microsatellite markers for Schistosoma mansoni were developed using four genomic microsatellite-enriched libraries. Microsatellites were observed in 65.4% of all sequences. Primer pairs were designed and tested for 23 loci. Eighteen loci produced amplification products, out of which 11 were polymorphic and were further characterized on 100 individuals of S. mansoni. Two to 19 alleles per locus were detected. The average values of expected and observed heterozygosities among the 11 loci were 0.79 and 0.59, respectively.

KW - Computational biology

KW - Genome

KW - Genomic library

KW - Microsatellites

KW - Population genetics

KW - Schistosoma mansoni

UR - https://www.scopus.com/pages/publications/33847373358

U2 - 10.1111/j.1471-8286.2006.01575.x

DO - 10.1111/j.1471-8286.2006.01575.x

M3 - Review article

AN - SCOPUS:33847373358

SN - 1471-8278

VL - 7

SP - 263

EP - 265

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 2

ER -

Microsatellite-enriched genomic libraries as a source of polymorphic loci for Schistosoma mansoni: Primer note

Abstract

Keywords

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