Abstract
The maintenance of cell volume is critical to health. Cell volume change reflects many biological and physiological processes. We have developed a lab-chip to measure cell volume change in real-time with high sensitivity and resolution, and applicable to both adherent and suspended cell populations. The volume change was detected by measuring the impedance of extra-cellular solution within a microfluidic chamber containing the cells. Using microfabrication to make precise chamber dimensions, volume change can be detected in response to an osmotic gradient <1mOsm. The sensor provides rapid screening of pharmaceutical agents affecting cell volume. We have screened for peptides that affect cell volume regulation and found one in spider venom that inhibits at ∼100pM.
| Original language | English |
|---|---|
| Article number | AA5.12 |
| Pages (from-to) | 261-266 |
| Number of pages | 6 |
| Journal | Materials Research Society Symposium - Proceedings |
| Volume | 845 |
| State | Published - 2005 |
| Event | Nanoscale Materials Science in Biology and Medicine - Boston, MA, United States Duration: Nov 28 2004 → Dec 2 2004 |
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