Micro-spectroscopy of chloroplasts in protoplasts from Arabidopsis thaliana under single- and multi-photon excitations

Fu Jen Kao; Yi Min Wang; Jian Cheng Chen; Ping Chin Cheng; Rung Wu Chen; Bai Ling Lin

doi:10.1016/S0022-2313(02)00258-2

Micro-spectroscopy of chloroplasts in protoplasts from Arabidopsis thaliana under single- and multi-photon excitations

Fu Jen Kao
, Yi Min Wang
, Jian Cheng Chen
, Ping Chin Cheng
, Rung Wu Chen
, Bai Ling Lin

Department of Electrical Engineering

National Sun Yat-sen University
Academia Sinica - Institute of Molecular Biology

Research output: Contribution to journal › Conference article › peer-review

7 Scopus citations

Abstract

Ultrafast laser pulses are known to generate impacts on specimens in ways much different from cw illumination in the applications of confocal microscopy. In this study, protoplasts from Arabidopsis thaliana are employed as the samples to compare the effects induced by single- and multi-photon excitations under confocal imaging configuration. Through time-lapsed micro-spectroscopy we are able to characterize the differences in photo-bleaching and the evolution of fluorescence spectra of chloroplasts thus induced. These differences may be attributed to the disruption of chained reaction as a result of multi-photon excitation in photosynthesis.

Original language	English
Pages (from-to)	107-114
Number of pages	8
Journal	Journal of Luminescence
Volume	98
Issue number	1-4
DOIs	https://doi.org/10.1016/S0022-2313(02)00258-2
State	Published - Jul 2002
Event	HBSM 2001 - Taipei, Taiwan, Province of China Duration: Nov 18 2001 → Nov 23 2001

Keywords

Chloroplasts
Micro-spectroscopy
Multi-photon excitation
Photo-bleaching

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10.1016/S0022-2313(02)00258-2

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title = "Micro-spectroscopy of chloroplasts in protoplasts from Arabidopsis thaliana under single- and multi-photon excitations",

abstract = "Ultrafast laser pulses are known to generate impacts on specimens in ways much different from cw illumination in the applications of confocal microscopy. In this study, protoplasts from Arabidopsis thaliana are employed as the samples to compare the effects induced by single- and multi-photon excitations under confocal imaging configuration. Through time-lapsed micro-spectroscopy we are able to characterize the differences in photo-bleaching and the evolution of fluorescence spectra of chloroplasts thus induced. These differences may be attributed to the disruption of chained reaction as a result of multi-photon excitation in photosynthesis.",

keywords = "Chloroplasts, Micro-spectroscopy, Multi-photon excitation, Photo-bleaching",

author = "Kao, \{Fu Jen\} and Wang, \{Yi Min\} and Chen, \{Jian Cheng\} and Cheng, \{Ping Chin\} and Chen, \{Rung Wu\} and Lin, \{Bai Ling\}",

year = "2002",

month = jul,

doi = "10.1016/S0022-2313(02)00258-2",

language = "English",

volume = "98",

pages = "107--114",

journal = "Journal of Luminescence",

issn = "0022-2313",

publisher = "Elsevier B.V.",

number = "1-4",

note = "HBSM 2001 ; Conference date: 18-11-2001 Through 23-11-2001",

}

TY - JOUR

T1 - Micro-spectroscopy of chloroplasts in protoplasts from Arabidopsis thaliana under single- and multi-photon excitations

AU - Kao, Fu Jen

AU - Wang, Yi Min

AU - Chen, Jian Cheng

AU - Cheng, Ping Chin

AU - Chen, Rung Wu

AU - Lin, Bai Ling

PY - 2002/7

Y1 - 2002/7

N2 - Ultrafast laser pulses are known to generate impacts on specimens in ways much different from cw illumination in the applications of confocal microscopy. In this study, protoplasts from Arabidopsis thaliana are employed as the samples to compare the effects induced by single- and multi-photon excitations under confocal imaging configuration. Through time-lapsed micro-spectroscopy we are able to characterize the differences in photo-bleaching and the evolution of fluorescence spectra of chloroplasts thus induced. These differences may be attributed to the disruption of chained reaction as a result of multi-photon excitation in photosynthesis.

AB - Ultrafast laser pulses are known to generate impacts on specimens in ways much different from cw illumination in the applications of confocal microscopy. In this study, protoplasts from Arabidopsis thaliana are employed as the samples to compare the effects induced by single- and multi-photon excitations under confocal imaging configuration. Through time-lapsed micro-spectroscopy we are able to characterize the differences in photo-bleaching and the evolution of fluorescence spectra of chloroplasts thus induced. These differences may be attributed to the disruption of chained reaction as a result of multi-photon excitation in photosynthesis.

KW - Chloroplasts

KW - Micro-spectroscopy

KW - Multi-photon excitation

KW - Photo-bleaching

UR - https://www.scopus.com/pages/publications/0036648384

U2 - 10.1016/S0022-2313(02)00258-2

DO - 10.1016/S0022-2313(02)00258-2

M3 - Conference article

AN - SCOPUS:0036648384

SN - 0022-2313

VL - 98

SP - 107

EP - 114

JO - Journal of Luminescence

JF - Journal of Luminescence

IS - 1-4

T2 - HBSM 2001

Y2 - 18 November 2001 through 23 November 2001

ER -

Micro-spectroscopy of chloroplasts in protoplasts from Arabidopsis thaliana under single- and multi-photon excitations

Abstract

Keywords

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