Measurement of immunoglobulin concentration in cell culture supernates by computer-assisted ELISA

Herbert B. Slade; Dennis E. Lopatin; Stanley A. Schwartz

doi:10.1016/0022-1759(86)90230-9

Measurement of immunoglobulin concentration in cell culture supernates by computer-assisted ELISA

Herbert B. Slade
, Dennis E. Lopatin
, Stanley A. Schwartz

Medicine

University of Michigan, Ann Arbor

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The enzyme-linked immunosorbent assay (ELISA) is used extensively in immunologic research for obtaining quantitative estimates of immunoglobulin concentration in cell culture supernates. Through incorporation of a microcomputer for data acquisition, storage and rapid calculation of results, a substantial reduction in total assay time may be realized. Described here are a set of menu-driven programs written in Basic for the IBM-PC which provide advantages over existing software in simplicity, versatility and accuracy. Hardware requirements are minimal. These programs should encourage greater flexibility in terms of the size and complexity of experimental designs.

Original language	English
Pages (from-to)	169-179
Number of pages	11
Journal	Journal of Immunological Methods
Volume	94
Issue number	1-2
DOIs	https://doi.org/10.1016/0022-1759(86)90230-9
State	Published - Nov 20 1986

Keywords

Cell culture
Computer
ELISA
Immunoglobulin

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10.1016/0022-1759(86)90230-9

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abstract = "The enzyme-linked immunosorbent assay (ELISA) is used extensively in immunologic research for obtaining quantitative estimates of immunoglobulin concentration in cell culture supernates. Through incorporation of a microcomputer for data acquisition, storage and rapid calculation of results, a substantial reduction in total assay time may be realized. Described here are a set of menu-driven programs written in Basic for the IBM-PC which provide advantages over existing software in simplicity, versatility and accuracy. Hardware requirements are minimal. These programs should encourage greater flexibility in terms of the size and complexity of experimental designs.",

keywords = "Cell culture, Computer, ELISA, Immunoglobulin",

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AU - Slade, Herbert B.

AU - Lopatin, Dennis E.

AU - Schwartz, Stanley A.

PY - 1986/11/20

Y1 - 1986/11/20

N2 - The enzyme-linked immunosorbent assay (ELISA) is used extensively in immunologic research for obtaining quantitative estimates of immunoglobulin concentration in cell culture supernates. Through incorporation of a microcomputer for data acquisition, storage and rapid calculation of results, a substantial reduction in total assay time may be realized. Described here are a set of menu-driven programs written in Basic for the IBM-PC which provide advantages over existing software in simplicity, versatility and accuracy. Hardware requirements are minimal. These programs should encourage greater flexibility in terms of the size and complexity of experimental designs.

AB - The enzyme-linked immunosorbent assay (ELISA) is used extensively in immunologic research for obtaining quantitative estimates of immunoglobulin concentration in cell culture supernates. Through incorporation of a microcomputer for data acquisition, storage and rapid calculation of results, a substantial reduction in total assay time may be realized. Described here are a set of menu-driven programs written in Basic for the IBM-PC which provide advantages over existing software in simplicity, versatility and accuracy. Hardware requirements are minimal. These programs should encourage greater flexibility in terms of the size and complexity of experimental designs.

KW - Cell culture

KW - Computer

KW - ELISA

KW - Immunoglobulin

UR - https://www.scopus.com/pages/publications/0022976324

U2 - 10.1016/0022-1759(86)90230-9

DO - 10.1016/0022-1759(86)90230-9

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EP - 179

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

Measurement of immunoglobulin concentration in cell culture supernates by computer-assisted ELISA

Abstract

Keywords

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