@inproceedings{a838835a08684fb58015a5a4642106ec,
title = "Mapping of intracellular concentrations of macromolecules by two-photon excited fluorescence lifetime imaging",
abstract = "Measurements and monitoring of concentrations of macromolecules in live cells and sub-cellular structures is of tremendous interest in cell biology and translational medicine. In this report we demonstrate a breakthrough potential of FLIM for real-time quantitative mapping of macromolecular distribution in the cell. In our approach we exploit a correlation existing between the fluorescence lifetime of fluorophores, refractive index and local concentrations of cellular macromolecules in the of fluorophore's microenvironment. We show a value of our approach for fundamental cell science and cellular diagnostic assays.",
keywords = "fluorescence lifetime imaging microscopy (FLIM), macromolecule, protein concentration, refractive index, two-photon excited fluorescence",
author = "Lixin Liu and Artem Pliss and Xiao Peng and Andrey Kuzmin and Junle Qu and Prasad, \{Paras N.\}",
note = "Publisher Copyright: Copyright {\textcopyright} 2016 SPIE.; Multiphoton Microscopy in the Biomedical Sciences XVI ; Conference date: 14-02-2016 Through 16-02-2016",
year = "2016",
doi = "10.1117/12.2211889",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "So, \{Peter T. C.\} and Karsten Konig and Ammasi Periasamy and Karsten Konig",
booktitle = "Multiphoton Microscopy in the Biomedical Sciences XVI",
address = "United States",
}