Longitudinal assessment of peptide recoveries from a sample solution in an autosampler vial for proteomics

Relatively few studies on time-related peptide losses have been reported and previous studies on the differential peptide recovery from a sample solution over a period had limitations. Thus, here, longitudinal peptide losses during sample solution storage in an autosampler vial over 145 h were assessed using nanoliquid chromatography and tandem mass spectrometry. In this study, hydrophilic peptide losses from a solution stored in a vial were observed regardless of materials of vials and the possibility of hydrophobic peptide losses brought by reasons other than hydrophobic peptide adsorption onto vials was found. Additionally, the preparation of a proteomics sample using 0.1% aqueous formic acid solution (0.1% FA) and a polypropylene vial, 0.1% FA and a glass vial, or the aqueous mixture of 5Murea, 1Mthiourea, and 0.1% FA and a polymethylpentene vial proved to produce the most reliable longitudinal recovery of peptides from a solution stored in a vial.