Liquid chromatographic-mass spectrometric determination of endogenous γ-hydroxybutyrate concentrations in rat brain regions and plasma

Ho Leung Fung; Eric Haas; Joseph Raybon; Jian Xu; Sun Mi Fung

doi:10.1016/j.jchromb.2004.04.022

Liquid chromatographic-mass spectrometric determination of endogenous γ-hydroxybutyrate concentrations in rat brain regions and plasma

Ho Leung Fung
, Eric Haas
, Joseph Raybon
, Jian Xu
, Sun Mi Fung

Pharmaceutical Sciences

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

A new liquid chromatographic-mass spectrometric (LC-MS) method for determining trace concentrations of γ-hydroxybutyric acid (GHB) in biological samples has been developed. This method utilizes solid-phase extraction for separation, deuterated GHB as an internal standard (IS) and multiple reaction monitoring (MRM) in the negative ion mode to detect the parent and product ions (103 and 57 for GHB, and 109 and 61 for D6-GHB, respectively). The assay produces excellent linearity and reproducibility, with a limit of quantification (LOQ) of about 0.1 μg/ml. The method has been applied for the determination of endogenous GHB in various rat brain regions.

Original language	English
Pages (from-to)	287-291
Number of pages	5
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	807
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2004.04.022
State	Published - Aug 5 2004

Keywords

γ-Hydroxybutyrate

Access to Document

10.1016/j.jchromb.2004.04.022

Cite this

@article{436e345e23434ec68e78a00750c37805,

title = "Liquid chromatographic-mass spectrometric determination of endogenous γ-hydroxybutyrate concentrations in rat brain regions and plasma",

abstract = "A new liquid chromatographic-mass spectrometric (LC-MS) method for determining trace concentrations of γ-hydroxybutyric acid (GHB) in biological samples has been developed. This method utilizes solid-phase extraction for separation, deuterated GHB as an internal standard (IS) and multiple reaction monitoring (MRM) in the negative ion mode to detect the parent and product ions (103 and 57 for GHB, and 109 and 61 for D6-GHB, respectively). The assay produces excellent linearity and reproducibility, with a limit of quantification (LOQ) of about 0.1 μg/ml. The method has been applied for the determination of endogenous GHB in various rat brain regions.",

keywords = "γ-Hydroxybutyrate",

author = "Fung, \{Ho Leung\} and Eric Haas and Joseph Raybon and Jian Xu and Fung, \{Sun Mi\}",

year = "2004",

month = aug,

day = "5",

doi = "10.1016/j.jchromb.2004.04.022",

language = "English",

volume = "807",

pages = "287--291",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

publisher = "Elsevier B.V.",

number = "2",

}

Liquid chromatographic-mass spectrometric determination of endogenous γ-hydroxybutyrate concentrations in rat brain regions and plasma. / Fung, Ho Leung; Haas, Eric; Raybon, Joseph et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 807, No. 2, 05.08.2004, p. 287-291.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Liquid chromatographic-mass spectrometric determination of endogenous γ-hydroxybutyrate concentrations in rat brain regions and plasma

AU - Fung, Ho Leung

AU - Haas, Eric

AU - Raybon, Joseph

AU - Xu, Jian

AU - Fung, Sun Mi

PY - 2004/8/5

Y1 - 2004/8/5

N2 - A new liquid chromatographic-mass spectrometric (LC-MS) method for determining trace concentrations of γ-hydroxybutyric acid (GHB) in biological samples has been developed. This method utilizes solid-phase extraction for separation, deuterated GHB as an internal standard (IS) and multiple reaction monitoring (MRM) in the negative ion mode to detect the parent and product ions (103 and 57 for GHB, and 109 and 61 for D6-GHB, respectively). The assay produces excellent linearity and reproducibility, with a limit of quantification (LOQ) of about 0.1 μg/ml. The method has been applied for the determination of endogenous GHB in various rat brain regions.

AB - A new liquid chromatographic-mass spectrometric (LC-MS) method for determining trace concentrations of γ-hydroxybutyric acid (GHB) in biological samples has been developed. This method utilizes solid-phase extraction for separation, deuterated GHB as an internal standard (IS) and multiple reaction monitoring (MRM) in the negative ion mode to detect the parent and product ions (103 and 57 for GHB, and 109 and 61 for D6-GHB, respectively). The assay produces excellent linearity and reproducibility, with a limit of quantification (LOQ) of about 0.1 μg/ml. The method has been applied for the determination of endogenous GHB in various rat brain regions.

KW - γ-Hydroxybutyrate

UR - https://www.scopus.com/pages/publications/2942615356

U2 - 10.1016/j.jchromb.2004.04.022

DO - 10.1016/j.jchromb.2004.04.022

M3 - Article

C2 - 15203042

AN - SCOPUS:2942615356

SN - 1570-0232

VL - 807

SP - 287

EP - 291

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 2

ER -

Liquid chromatographic-mass spectrometric determination of endogenous γ-hydroxybutyrate concentrations in rat brain regions and plasma

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this