Ligand Incorporation into Protein Microcrystals for MicroED by On-Grid Soaking

Michael W. Martynowycz; Tamir Gonen

doi:10.1016/j.str.2020.09.003

Ligand Incorporation into Protein Microcrystals for MicroED by On-Grid Soaking

Michael W. Martynowycz
, Tamir Gonen

Department of Structural Biology

University of California at Los Angeles

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Microcrystals are soaked with a small molecule and machined to ideal thickness for structural investigation by MicroED. The structure of a macromolecular protein crystal in complex with ligands is solved using continuous rotation MicroED data collected from a single prepared crystalline lamella.

Original language	English
Pages (from-to)	88-95.e2
Journal	Structure
Volume	29
Issue number	1
DOIs	https://doi.org/10.1016/j.str.2020.09.003
State	Published - Jan 7 2021

Keywords

-SEM
MicroED
cryoelectron microscopy (cryo-EM)
drug discovery
focused ion-beam (FIB)
ligand soaking

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10.1016/j.str.2020.09.003

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title = "Ligand Incorporation into Protein Microcrystals for MicroED by On-Grid Soaking",

abstract = "Microcrystals are soaked with a small molecule and machined to ideal thickness for structural investigation by MicroED. The structure of a macromolecular protein crystal in complex with ligands is solved using continuous rotation MicroED data collected from a single prepared crystalline lamella.",

keywords = "-SEM, MicroED, cryoelectron microscopy (cryo-EM), drug discovery, focused ion-beam (FIB), ligand soaking",

author = "Martynowycz, \{Michael W.\} and Tamir Gonen",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2021",

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T1 - Ligand Incorporation into Protein Microcrystals for MicroED by On-Grid Soaking

AU - Martynowycz, Michael W.

AU - Gonen, Tamir

PY - 2021/1/7

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N2 - Microcrystals are soaked with a small molecule and machined to ideal thickness for structural investigation by MicroED. The structure of a macromolecular protein crystal in complex with ligands is solved using continuous rotation MicroED data collected from a single prepared crystalline lamella.

AB - Microcrystals are soaked with a small molecule and machined to ideal thickness for structural investigation by MicroED. The structure of a macromolecular protein crystal in complex with ligands is solved using continuous rotation MicroED data collected from a single prepared crystalline lamella.

KW - -SEM

KW - MicroED

KW - cryoelectron microscopy (cryo-EM)

KW - drug discovery

KW - focused ion-beam (FIB)

KW - ligand soaking

UR - https://www.scopus.com/pages/publications/85092663459

U2 - 10.1016/j.str.2020.09.003

DO - 10.1016/j.str.2020.09.003

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AN - SCOPUS:85092663459

SN - 0969-2126

VL - 29

SP - 88-95.e2

JO - Structure

JF - Structure

IS - 1

ER -

Ligand Incorporation into Protein Microcrystals for MicroED by On-Grid Soaking

Abstract

Keywords

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