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Label-free rapid detection of pathogens with antimicrobial peptide assisted impedance spectrometry

  • Keren Jiang
  • , Hashem Etayash
  • , Sarfuddin Azmi
  • , Garima Thakur
  • , Selvaraj Naicker
  • , Kamaljit Kaur
  • , Thundat Thomas
  • University of Alberta

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

An urgent need exist for developing handheld devices for rapid, sensitive, and specific detection method for pathogens. Here we demonstrate a rapid detection method for Gram-positive and Gram-negative bacteria using an impedance sensor array functionalized with antimicrobial peptides (AMPs). This impedance sensor screens pathogens in real-time and has comparable sensitivity with current detection methods like polymerase chain reaction (PCR) and immunoassay. Functionalized electrodes in array selectively bind to the corresponding bacteria strains, resulting in variations in the impedance modulus. Impedance variation is used to detect incubated bacterial cell concentration with a resolution of 1 cell \iL'1. The dynamic range of detection for both Gram-positive and Gram-negative bacteria is found to be 103-106 cm mL"1. Micropattemed electrodes modified with AMPs in an impedimetric array offer an excellent platform for rapid and selective detection of pathogens in contaminated water and food products.

Original languageEnglish
Title of host publicationFoundations of Bio/Nano Interfaces - Synthesis, Modeling, Design Principles and Applications
EditorsHendrik Heinz, Carole C. Perry, Candan Tamerler, Yuhei Hayamizu
PublisherMaterials Research Society
Pages13-18
Number of pages6
ISBN (Electronic)9781510826465
DOIs
StatePublished - 2015
Event2015 MRS Spring Meeting - San Francisco, United States
Duration: Apr 6 2015Apr 10 2015

Publication series

NameMaterials Research Society Symposium Proceedings
Volume1793
ISSN (Print)0272-9172

Conference

Conference2015 MRS Spring Meeting
Country/TerritoryUnited States
CitySan Francisco
Period04/6/1504/10/15

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