Klebsiella pneumoniae ST147: and then there were three carbapenemases

L. F. Mataseje; L. Chen; G. Peirano; K. Fakharuddin; B. Kreiswith; M. Mulvey; J. D.D. Pitout

doi:10.1007/s10096-022-04514-4

Klebsiella pneumoniae ST147: and then there were three carbapenemases

L. F. Mataseje
, L. Chen
, G. Peirano
, K. Fakharuddin
, B. Kreiswith
, M. Mulvey
, J. D.D. Pitout

Pharmacy

Public Health Agency of Canada
Alberta Precision Laboratories
University of Calgary
Hackensack Meridian Health
Seton Hall University
University of Pretoria

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Gram-negative bacteria containing three different carbapenemases are extremely rare. Klebsiella pneumoniae (N22-925) with KPC-2, NDM-1, and OXA-48 was obtained from a Canadian patient with recent hospitalization in Romania. Short and long read whole genome sequencing showed that the bla_KPC-2 was situated on a 214 kb IncFIB(K)/IncFII(K) plasmid, the bla_NDM-1 on a 104 kb IncFIB (pQil)/IncFII(K) plasmid, and the bla_OXA-48 on a 64 kb IncL plasmid. These plasmids were conjugated to Escherichia coli J53. N22-925 belonged to a unique ST147 cluster that is likely endemic in Romania. This case emphasizes the need for rapid carbapenemase screening in patients from endemic regions. We described the first complete genome sequence of a K. pneumoniae isolate with three different carbapenemases, providing a reference for future studies on this rarely reported occurrence.

Original language	English
Pages (from-to)	1467-1472
Number of pages	6
Journal	European Journal of Clinical Microbiology and Infectious Diseases
Volume	41
Issue number	12
DOIs	https://doi.org/10.1007/s10096-022-04514-4
State	Published - Dec 2022

Keywords

Bla
Bla
Bla
K. pneumoniae ST147

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title = "Klebsiella pneumoniae ST147: and then there were three carbapenemases",

abstract = "Gram-negative bacteria containing three different carbapenemases are extremely rare. Klebsiella pneumoniae (N22-925) with KPC-2, NDM-1, and OXA-48 was obtained from a Canadian patient with recent hospitalization in Romania. Short and long read whole genome sequencing showed that the blaKPC-2 was situated on a 214 kb IncFIB(K)/IncFII(K) plasmid, the blaNDM-1 on a 104 kb IncFIB (pQil)/IncFII(K) plasmid, and the blaOXA-48 on a 64 kb IncL plasmid. These plasmids were conjugated to Escherichia coli J53. N22-925 belonged to a unique ST147 cluster that is likely endemic in Romania. This case emphasizes the need for rapid carbapenemase screening in patients from endemic regions. We described the first complete genome sequence of a K. pneumoniae isolate with three different carbapenemases, providing a reference for future studies on this rarely reported occurrence.",

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note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.",

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T2 - and then there were three carbapenemases

AU - Mataseje, L. F.

AU - Chen, L.

AU - Peirano, G.

AU - Fakharuddin, K.

AU - Kreiswith, B.

AU - Mulvey, M.

AU - Pitout, J. D.D.

PY - 2022/12

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N2 - Gram-negative bacteria containing three different carbapenemases are extremely rare. Klebsiella pneumoniae (N22-925) with KPC-2, NDM-1, and OXA-48 was obtained from a Canadian patient with recent hospitalization in Romania. Short and long read whole genome sequencing showed that the blaKPC-2 was situated on a 214 kb IncFIB(K)/IncFII(K) plasmid, the blaNDM-1 on a 104 kb IncFIB (pQil)/IncFII(K) plasmid, and the blaOXA-48 on a 64 kb IncL plasmid. These plasmids were conjugated to Escherichia coli J53. N22-925 belonged to a unique ST147 cluster that is likely endemic in Romania. This case emphasizes the need for rapid carbapenemase screening in patients from endemic regions. We described the first complete genome sequence of a K. pneumoniae isolate with three different carbapenemases, providing a reference for future studies on this rarely reported occurrence.

AB - Gram-negative bacteria containing three different carbapenemases are extremely rare. Klebsiella pneumoniae (N22-925) with KPC-2, NDM-1, and OXA-48 was obtained from a Canadian patient with recent hospitalization in Romania. Short and long read whole genome sequencing showed that the blaKPC-2 was situated on a 214 kb IncFIB(K)/IncFII(K) plasmid, the blaNDM-1 on a 104 kb IncFIB (pQil)/IncFII(K) plasmid, and the blaOXA-48 on a 64 kb IncL plasmid. These plasmids were conjugated to Escherichia coli J53. N22-925 belonged to a unique ST147 cluster that is likely endemic in Romania. This case emphasizes the need for rapid carbapenemase screening in patients from endemic regions. We described the first complete genome sequence of a K. pneumoniae isolate with three different carbapenemases, providing a reference for future studies on this rarely reported occurrence.

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JO - European Journal of Clinical Microbiology and Infectious Diseases

JF - European Journal of Clinical Microbiology and Infectious Diseases

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ER -

Klebsiella pneumoniae ST147: and then there were three carbapenemases

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Keywords

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