Abstract
Isolated nonperfused proximal tubules maintained their cell volume at a constant level (isovolumetric regulation, IVR), when osmolality of the bathing medium was gradually decreased from 290 to 190 mosm at 1.5 and 5.0 mosm/min. Hypotonic IVR was blocked by inhibiting the Na+-K+ pump with ouabain (10-4 M) when osmolality was decreased at 1.5 or 5mosm/min. Concentration-dependent inhibition of cell volume maintenance was obseved in the presence of the K+ channel blocker barium (10-3-10-2 M) when osmolality decreased at 5 mosm/min. Quinine (10-3 M), another K+ channel blocker, also inhibited IVR at osmolality decreases of 1.5 and 5 mosm/min. These results suggest that the maintenance of constant cell volume during gradual hypoosmotic exposure involves mechanisms that depend on intact Na-K-ATPase and the controlled loss of intracellular K+.
| Original language | English |
|---|---|
| Pages (from-to) | 233-240 |
| Number of pages | 8 |
| Journal | Renal Physiology and Biochemistry |
| Volume | 13 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1990 |
Keywords
- Cell volume regulation
- Hypoosmality
- Ouabain
- Potassium
- Proximal renal tubules
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