Isolation, sequencing, and characterization of crp-5, a gene encoding a Neurospora ribosomal protein

Zihua Wang; Khaled A. Tarawneh; Stephen J. Free

doi:10.1007/BF00310894

Isolation, sequencing, and characterization of crp-5, a gene encoding a Neurospora ribosomal protein

Zihua Wang
, Khaled A. Tarawneh
, Stephen J. Free

Department of Biological Sciences

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A Neurospora crassa cytoplasmic ribosomal protein gene, crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolate crp-5 genomic sequences. Computer analysis of the DNA sequences showed that they contain an open reading frame which encodes a protein homologous to the rat ribosomal protein S26. The crp-5 mRNA levels are regulated in a carbon-source-dependent manner. The organization of the gene and the region upstream of the coding sequences are discussed.

Original language	English
Pages (from-to)	330-333
Number of pages	4
Journal	Current Genetics
Volume	23
Issue number	4
DOIs	https://doi.org/10.1007/BF00310894
State	Published - Apr 1993

Keywords

Neurospora crassa
Ribosomal protein gene
crp-5

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10.1007/BF00310894

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abstract = "A Neurospora crassa cytoplasmic ribosomal protein gene, crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolate crp-5 genomic sequences. Computer analysis of the DNA sequences showed that they contain an open reading frame which encodes a protein homologous to the rat ribosomal protein S26. The crp-5 mRNA levels are regulated in a carbon-source-dependent manner. The organization of the gene and the region upstream of the coding sequences are discussed.",

keywords = "Neurospora crassa, Ribosomal protein gene, crp-5",

author = "Zihua Wang and Tarawneh, \{Khaled A.\} and Free, \{Stephen J.\}",

year = "1993",

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AU - Wang, Zihua

AU - Tarawneh, Khaled A.

AU - Free, Stephen J.

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AB - A Neurospora crassa cytoplasmic ribosomal protein gene, crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolate crp-5 genomic sequences. Computer analysis of the DNA sequences showed that they contain an open reading frame which encodes a protein homologous to the rat ribosomal protein S26. The crp-5 mRNA levels are regulated in a carbon-source-dependent manner. The organization of the gene and the region upstream of the coding sequences are discussed.

KW - Neurospora crassa

KW - Ribosomal protein gene

KW - crp-5

UR - https://www.scopus.com/pages/publications/0027537096

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EP - 333

JO - Current Genetics

JF - Current Genetics

IS - 4

ER -

Isolation, sequencing, and characterization of crp-5, a gene encoding a Neurospora ribosomal protein

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Keywords

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