Isolation of lactose-binding lectins from axolotl (Ambystoma mexicanum)

Howard J. Allen; Hafiz Ahmed; Ashu Sharma

doi:10.1016/0305-0491(92)90297-5

Isolation of lactose-binding lectins from axolotl (Ambystoma mexicanum)

Howard J. Allen
, Hafiz Ahmed
, Ashu Sharma

Department of Oral Biology

Roswell Park Cancer Institute

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

1. 1. Lactose-inhibitable hemagglutination activity was identified in extracts of axolotl (Ambystoma mexicanum) larvae. 2. 2. Two types of lectin were isolated from extracts by affinity chromatography on lactose-Sepharose. 3. 3. A thiol-independent lectin of subunit mol. wt 15 kDa and a thiol-dependent lectin of subunit mol. wt 18 kDa were identified. 4. 4. The 15 kDa and a 18 kDa polypeptides were weakly reactive with polyclonal anti-human galaptin serum.

Original language	English
Pages (from-to)	313-315
Number of pages	3
Journal	Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Volume	103
Issue number	2
DOIs	https://doi.org/10.1016/0305-0491(92)90297-5
State	Published - Oct 1992

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title = "Isolation of lactose-binding lectins from axolotl (Ambystoma mexicanum)",

abstract = "1. 1. Lactose-inhibitable hemagglutination activity was identified in extracts of axolotl (Ambystoma mexicanum) larvae. 2. 2. Two types of lectin were isolated from extracts by affinity chromatography on lactose-Sepharose. 3. 3. A thiol-independent lectin of subunit mol. wt 15 kDa and a thiol-dependent lectin of subunit mol. wt 18 kDa were identified. 4. 4. The 15 kDa and a 18 kDa polypeptides were weakly reactive with polyclonal anti-human galaptin serum.",

author = "Allen, \{Howard J.\} and Hafiz Ahmed and Ashu Sharma",

year = "1992",

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doi = "10.1016/0305-0491(92)90297-5",

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journal = "Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology",

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T1 - Isolation of lactose-binding lectins from axolotl (Ambystoma mexicanum)

AU - Allen, Howard J.

AU - Ahmed, Hafiz

AU - Sharma, Ashu

PY - 1992/10

Y1 - 1992/10

N2 - 1. 1. Lactose-inhibitable hemagglutination activity was identified in extracts of axolotl (Ambystoma mexicanum) larvae. 2. 2. Two types of lectin were isolated from extracts by affinity chromatography on lactose-Sepharose. 3. 3. A thiol-independent lectin of subunit mol. wt 15 kDa and a thiol-dependent lectin of subunit mol. wt 18 kDa were identified. 4. 4. The 15 kDa and a 18 kDa polypeptides were weakly reactive with polyclonal anti-human galaptin serum.

AB - 1. 1. Lactose-inhibitable hemagglutination activity was identified in extracts of axolotl (Ambystoma mexicanum) larvae. 2. 2. Two types of lectin were isolated from extracts by affinity chromatography on lactose-Sepharose. 3. 3. A thiol-independent lectin of subunit mol. wt 15 kDa and a thiol-dependent lectin of subunit mol. wt 18 kDa were identified. 4. 4. The 15 kDa and a 18 kDa polypeptides were weakly reactive with polyclonal anti-human galaptin serum.

UR - https://www.scopus.com/pages/publications/0026662759

U2 - 10.1016/0305-0491(92)90297-5

DO - 10.1016/0305-0491(92)90297-5

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AN - SCOPUS:0026662759

SN - 1096-4959

VL - 103

SP - 313

EP - 315

JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology

IS - 2

ER -

Isolation of lactose-binding lectins from axolotl (Ambystoma mexicanum)

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