Isolation and characterization of microsatellite loci in the Caribbean gorgonian Pseudopterogorgia elisabethae

Carla Gutiérrez-Rodríguez; Howard R. Lasker

doi:10.1111/j.1471-8286.2004.00595.x

Isolation and characterization of microsatellite loci in the Caribbean gorgonian Pseudopterogorgia elisabethae

Carla Gutiérrez-Rodríguez
, Howard R. Lasker

SUNY Buffalo
Ohio University

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

We report on the isolation and characterization of five polymorphic microsatellites in the gorgonian Pseudopterogorgia elisabethae from genomic DNA-enriched libraries. Forty-four microsatellites were screened from the libraries with the oligonucleotide probe (CA)₁₂. Five of the screened microsatellites were polymorphic. The levels of polymorphism found in 50 genotyped individuals from a single population suggest that microsatellites are useful tools for the study of genetic variation in gorgonians. These are the first microsatellite loci reported from any gorgonian species.

Original language	English
Pages (from-to)	153-155
Number of pages	3
Journal	Molecular Ecology Notes
Volume	4
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-8286.2004.00595.x
State	Published - Jun 2004

Keywords

Gorgonians
Microsatellites
Octocorallia
Polymorphism
Population genetics
Pseudopterogorgia elisabethae

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10.1111/j.1471-8286.2004.00595.x

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title = "Isolation and characterization of microsatellite loci in the Caribbean gorgonian Pseudopterogorgia elisabethae",

abstract = "We report on the isolation and characterization of five polymorphic microsatellites in the gorgonian Pseudopterogorgia elisabethae from genomic DNA-enriched libraries. Forty-four microsatellites were screened from the libraries with the oligonucleotide probe (CA)12. Five of the screened microsatellites were polymorphic. The levels of polymorphism found in 50 genotyped individuals from a single population suggest that microsatellites are useful tools for the study of genetic variation in gorgonians. These are the first microsatellite loci reported from any gorgonian species.",

keywords = "Gorgonians, Microsatellites, Octocorallia, Polymorphism, Population genetics, Pseudopterogorgia elisabethae",

author = "Carla Guti{\'e}rrez-Rodr{\'i}guez and Lasker, \{Howard R.\}",

year = "2004",

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AU - Gutiérrez-Rodríguez, Carla

AU - Lasker, Howard R.

PY - 2004/6

Y1 - 2004/6

N2 - We report on the isolation and characterization of five polymorphic microsatellites in the gorgonian Pseudopterogorgia elisabethae from genomic DNA-enriched libraries. Forty-four microsatellites were screened from the libraries with the oligonucleotide probe (CA)12. Five of the screened microsatellites were polymorphic. The levels of polymorphism found in 50 genotyped individuals from a single population suggest that microsatellites are useful tools for the study of genetic variation in gorgonians. These are the first microsatellite loci reported from any gorgonian species.

AB - We report on the isolation and characterization of five polymorphic microsatellites in the gorgonian Pseudopterogorgia elisabethae from genomic DNA-enriched libraries. Forty-four microsatellites were screened from the libraries with the oligonucleotide probe (CA)12. Five of the screened microsatellites were polymorphic. The levels of polymorphism found in 50 genotyped individuals from a single population suggest that microsatellites are useful tools for the study of genetic variation in gorgonians. These are the first microsatellite loci reported from any gorgonian species.

KW - Gorgonians

KW - Microsatellites

KW - Octocorallia

KW - Polymorphism

KW - Population genetics

KW - Pseudopterogorgia elisabethae

UR - https://www.scopus.com/pages/publications/2542498029

U2 - 10.1111/j.1471-8286.2004.00595.x

DO - 10.1111/j.1471-8286.2004.00595.x

M3 - Article

AN - SCOPUS:2542498029

SN - 1471-8278

VL - 4

SP - 153

EP - 155

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 2

ER -

Isolation and characterization of microsatellite loci in the Caribbean gorgonian Pseudopterogorgia elisabethae

Abstract

Keywords

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