ISKpn40-Mediated Mobilization of the colistin resistance gene mcr-3.11 in Escherichia coli

Yu Zhang He; Teng Fei Long; Cai Ping Chen; Bing He; Xing Ping Li; Jessica Schuyler; Liang Chen; Xiao Ping Liao; Ya Hong Liu; Jian Suna

doi:10.1128/AAC.00851-20

ISKpn40-Mediated Mobilization of the colistin resistance gene mcr-3.11 in Escherichia coli

Yu Zhang He
, Teng Fei Long
, Cai Ping Chen
, Bing He
, Xing Ping Li
, Jessica Schuyler
, Liang Chen
, Xiao Ping Liao
, Ya Hong Liu
, Jian Suna

Pharmacy

South China Agricultural University
Guangdong Laboratory for Lingnan Modern Agriculture
Hackensack Meridian Health
Yangzhou University

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The mobile colistin resistance gene mcr-3 has globally disseminated since it was first reported in 2017 in Escherichia coli. In vitro mobilization assays in this study demonstrate the functionality of the composite transposon structure ISKpn40-mcr-3.11-dgkA-ISKpn40 in wild-type and recA E. coli strains. These transpositions generated 4-bp duplications at the target sites. This is the first report demonstrating the mobility of the mcr-3.11 gene by transposition.

Original language	English
Article number	e00851
Journal	Antimicrobial Agents and Chemotherapy
Volume	64
Issue number	10
DOIs	https://doi.org/10.1128/AAC.00851-20
State	Published - Oct 2020

Keywords

Colistin resistance
ISKpn40
Mcr-3.11
Transposition
Transposition mechanism

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10.1128/AAC.00851-20

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title = "ISKpn40-Mediated Mobilization of the colistin resistance gene mcr-3.11 in Escherichia coli",

abstract = "The mobile colistin resistance gene mcr-3 has globally disseminated since it was first reported in 2017 in Escherichia coli. In vitro mobilization assays in this study demonstrate the functionality of the composite transposon structure ISKpn40-mcr-3.11-dgkA-ISKpn40 in wild-type and recA E. coli strains. These transpositions generated 4-bp duplications at the target sites. This is the first report demonstrating the mobility of the mcr-3.11 gene by transposition.",

keywords = "Colistin resistance, ISKpn40, Mcr-3.11, Transposition, Transposition mechanism",

author = "He, \{Yu Zhang\} and Long, \{Teng Fei\} and Chen, \{Cai Ping\} and Bing He and Li, \{Xing Ping\} and Jessica Schuyler and Liang Chen and Liao, \{Xiao Ping\} and Liu, \{Ya Hong\} and Jian Suna",

year = "2020",

month = oct,

doi = "10.1128/AAC.00851-20",

language = "English",

volume = "64",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

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TY - JOUR

T1 - ISKpn40-Mediated Mobilization of the colistin resistance gene mcr-3.11 in Escherichia coli

AU - He, Yu Zhang

AU - Long, Teng Fei

AU - Chen, Cai Ping

AU - He, Bing

AU - Li, Xing Ping

AU - Schuyler, Jessica

AU - Chen, Liang

AU - Liao, Xiao Ping

AU - Liu, Ya Hong

AU - Suna, Jian

PY - 2020/10

Y1 - 2020/10

N2 - The mobile colistin resistance gene mcr-3 has globally disseminated since it was first reported in 2017 in Escherichia coli. In vitro mobilization assays in this study demonstrate the functionality of the composite transposon structure ISKpn40-mcr-3.11-dgkA-ISKpn40 in wild-type and recA E. coli strains. These transpositions generated 4-bp duplications at the target sites. This is the first report demonstrating the mobility of the mcr-3.11 gene by transposition.

AB - The mobile colistin resistance gene mcr-3 has globally disseminated since it was first reported in 2017 in Escherichia coli. In vitro mobilization assays in this study demonstrate the functionality of the composite transposon structure ISKpn40-mcr-3.11-dgkA-ISKpn40 in wild-type and recA E. coli strains. These transpositions generated 4-bp duplications at the target sites. This is the first report demonstrating the mobility of the mcr-3.11 gene by transposition.

KW - Colistin resistance

KW - ISKpn40

KW - Mcr-3.11

KW - Transposition

KW - Transposition mechanism

UR - https://www.scopus.com/pages/publications/85091469938

U2 - 10.1128/AAC.00851-20

DO - 10.1128/AAC.00851-20

M3 - Article

C2 - 32660996

AN - SCOPUS:85091469938

SN - 0066-4804

VL - 64

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 10

M1 - e00851

ER -

ISKpn40-Mediated Mobilization of the colistin resistance gene mcr-3.11 in Escherichia coli

Abstract

Keywords

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